Jaakkola Lassi, Peuralahti Jari, Hakala Harri, Mukkala Veli-Matti, Hurskainen Pertti, Hovinen Jari
PerkinElmer Life and Analytical Sciences, Turku Site, P.O. Box 10, FIN-20101 Turku, Finland.
J Pept Sci. 2006 Mar;12(3):199-205. doi: 10.1002/psc.697.
Synthesis of a building block that allows introduction of photoluminescent europium(III) and samarium(III) chelates to synthetic oligopeptides on solid phase using standard Fmoc chemistry is described. Upon completion of the oligopeptide synthesis, these conjugates were converted to the corresponding lanthanide(III) chelates by treatment with appropriate lanthanide(III) salt. Also synthesis of a new terpyridine-based europium(III) chelate designed for solution phase protein labeling is demonstrated.
本文描述了一种构建模块的合成方法，该方法可利用标准的Fmoc化学方法在固相上将光致发光的铕（III）和钐（III）螯合物引入合成寡肽中。寡肽合成完成后，通过用适当的镧系元素（III）盐处理，将这些缀合物转化为相应的镧系元素（III）螯合物。此外，还展示了一种设计用于溶液相蛋白质标记的新型基于三联吡啶的铕（III）螯合物的合成。
