用发光镧系（III）螯合物标记的寡核苷酸的固相合成。

Solid-phase synthesis of oligonucleotides labeled with luminescent lanthanide(III) chelates.

作者信息

Jaakkola Lassi, Peuralahti Jari, Hakala Harri, Kunttu Jenni, Tallqvist Pekka, Mukkala Veli-Matti, Ylikoski Alice, Hovinen Jari

机构信息

PerkinElmer Life and Analytical Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.

出版信息

Bioconjug Chem. 2005 May-Jun;16(3):700-9. doi: 10.1021/bc050019x.

DOI:10.1021/bc050019x

PMID:15898740

Abstract

The synthesis of phosphoramidite building blocks that allow introduction of luminescent europium(III), terbium(III), dysprosium(III), and samarium(III) chelates to oligonucleotides on the solid phase is described. Several labeled oligonucleotides using these building blocks were prepared, and the photophysical properties of these bioconjugates were investigated.

摘要

本文描述了亚磷酰胺砌块的合成方法，该方法可在固相上将发光的铕（III）、铽（III）、镝（III）和钐（III）螯合物引入寡核苷酸中。使用这些砌块制备了几种标记的寡核苷酸，并研究了这些生物共轭物的光物理性质。