Oxley Andrew P A, McKay David B
National Marine Science Centre, Coffs Harbour, New South Wales 2450, Australia.
Dis Aquat Organ. 2005 Jun;65(2):99-105. doi: 10.3354/dao065099.
Helicobacter species are widely distributed in the gastrointestinal system of humans and many animal taxa. Investigations of natural infections are essential to elucidating their role within the host. The feces of fur seals Arctocephalus pusillus doriferus and sea lions Neophoca cinerea from 3 separate captive populations, as well as a wild colony from Kangaroo Island, Australia, were examined for the occurrence of Helicobacter spp. The feces from several wild silver gulls Larus novahollandiae were also investigated. As detected by PCR, 18 of 21 samples from captive and 12 of 16 samples from wild seals were positive for Helicobacter spp. Three species were identified in these animals. Whilst one possibly novel type was identified from wild fur seals, the majority of wild and captive individuals had the same species. This species also occurred in more than 1 seal type and in silver gulls, and shared a 98.1 to 100% identity to other Helicobacter spp. from harp seals and sea otters. A similar sequence type to species identified from cetaceans was also detected in several captive seals. This study reports for the first time the presence of Helicobacter spp. in wild and captive seals and demonstrates the diversity and broad-host range of these organisms in the marine host.
幽门螺杆菌广泛分布于人类和许多动物类群的胃肠道系统中。对自然感染情况进行调查对于阐明它们在宿主体内的作用至关重要。对来自3个不同圈养种群的毛皮海狮（Arctocephalus pusillus doriferus）和海狗（Neophoca cinerea）以及澳大利亚袋鼠岛一个野生群体的粪便进行了检查，以确定幽门螺杆菌的存在情况。还对几只野生银鸥（Larus novahollandiae）的粪便进行了调查。通过聚合酶链反应（PCR）检测，圈养海豹的21份样本中有18份、野生海豹的16份样本中有12份幽门螺杆菌检测呈阳性。在这些动物中鉴定出了3个菌种。虽然从野生毛皮海狮中鉴定出了一种可能的新型菌种，但大多数野生和圈养个体具有相同的菌种。该菌种也存在于不止一种海豹类型以及银鸥中，并且与来自竖琴海豹和海獭的其他幽门螺杆菌菌种具有98.1%至100%的同源性。在几只圈养海豹中还检测到了与从鲸类动物中鉴定出的菌种相似的序列类型。本研究首次报告了野生和圈养海豹中存在幽门螺杆菌，并证明了这些微生物在海洋宿主中的多样性和广泛宿主范围。
