School of Resources and Environmental Engineering, Anhui University, Hefei, China.
Shengjin Lake National Nature Reserve of Anhui Province, Chizhou, China.
Microbiologyopen. 2017 Jun;6(3). doi: 10.1002/mbo3.447. Epub 2017 Jan 26.
Gut microbes of animals play critical roles in processes such as digestion and immunity. Therefore, identifying gut microbes will shed light on understanding the annual life of animal species, particularly those that are threatened or endangered. In the present study, we conducted nucleotide sequence analyses of the 16S rRNA genes of gut microbiome of the hooded cranes (Grus monacha) wintering at Shengjin Lake, China, by Illumina high-throughput sequencing technology. We acquired 503,398 high-quality sequences and 785 operational taxonomic units (OTUs) from 15 fecal samples from different cranes, representing 22 phyla that were dominated by Firmicutes, Proteobacteria, and Actinobacteria. A total of 305 genera were identified that were dominated by Clostridium, Lysinibacillus, and Enterobacter. The core gut microbiome comprised 26 genera, including many probiotic species such as Clostridium, Bacillus, Cellulosilyticum, and Cellulomonas that could catabolize cellulose. The findings reported here contribute to our knowledge of the microbiology of hooded cranes and will likely advance efforts to protect waterbirds that inhabit Shengjin Lake Reserve during winter.
动物肠道微生物在消化和免疫等过程中发挥着关键作用。因此，鉴定肠道微生物将有助于了解动物物种的年生活史，特别是那些受到威胁或濒危的物种。本研究采用 Illumina 高通量测序技术，对中国升金湖越冬的白头鹤（Grus monacha）肠道微生物的 16S rRNA 基因进行了核苷酸序列分析。我们从 15 个不同鹤的粪便样本中获得了 503398 条高质量序列和 785 个操作分类单元（OTUs），代表了 22 个门，其中厚壁菌门、变形菌门和放线菌门占优势。共鉴定出 305 个属，其中以梭菌属、赖氨酸芽孢杆菌属和肠杆菌属为主。核心肠道微生物群由 26 个属组成，其中包括许多可代谢纤维素的益生菌物种，如梭菌属、芽孢杆菌属、纤维分解菌属和纤维单胞菌属。本研究结果有助于我们了解白头鹤的微生物学，并可能有助于保护冬季栖息在升金湖保护区的水鸟。
