用于3D组织细胞培养的设计型自组装肽纳米纤维支架

Designer self-assembling peptide nanofiber scaffolds for 3D tissue cell cultures.

作者信息

Zhang Shuguang, Gelain Fabrizio, Zhao Xiaojun

机构信息

Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA.

出版信息

Semin Cancer Biol. 2005 Oct;15(5):413-20. doi: 10.1016/j.semcancer.2005.05.007.

DOI:10.1016/j.semcancer.2005.05.007

PMID:16061392

Abstract

Biomedical researchers have become increasingly aware of the limitations of time-honored conventional 2D tissue cell cultures where most tissue cell studies have been carried out. They are now searching for 3D cell culture systems, something between a petri dish and a mouse. It has become apparent that 3D cell culture offers a more realistic micro- and local-environment where the functional properties of cells can be observed and manipulated that is not possible in animals. A newly designer self-assembling peptide scaffolds may provide an ideally alternative system. The important implications of 3D tissue cell cultures for basic cell biology, tumor biology, high-content drug screening, and regenerative medicine and beyond could be profound.

摘要

生物医学研究人员越来越意识到长期以来进行大多数组织细胞研究的传统二维组织细胞培养的局限性。他们现在正在寻找三维细胞培养系统，一种介于培养皿和小鼠之间的东西。很明显，三维细胞培养提供了一个更现实的微观和局部环境，在这个环境中可以观察和操纵细胞的功能特性，而这在动物体内是不可能的。一种新设计的自组装肽支架可能提供一个理想的替代系统。三维组织细胞培养对基础细胞生物学、肿瘤生物学、高内涵药物筛选、再生医学及其他领域的重要意义可能是深远的。