Gao Yu, Su Di, Zhao Jiawei, Luo Zhongli, Lin Xuemei
College of Basic Medical Science, Chongqing Medical University, Chongqing 400016, China.
Molecular Medicine and Cancer Research Center, College of Basic Medical Science, Chongqing Medical University, Chongqing 400016, China.
Gels. 2025 May 27;11(6):394. doi: 10.3390/gels11060394.
In recent years, the incidence of colorectal cancer has been increasing annually. During the research on tumor treatment strategies, the translation of fundamental research findings into clinical applications has often been constrained by the limitations of existing tumor models, with few breakthroughs achieved to date. Therefore, our objective is to explore the feasibility of utilizing self-assembling short peptides (SAPs) to construct a three-dimensional (3D) culture system for colorectal adenocarcinoma cells for anticancer drug screening. By characterizing the physicochemical properties and biocompatibility of SAP SCIOBIO III, we demonstrated that it can rapidly self-assemble into a nanofibrous scaffold under ionic triggers, supporting 3D cell culture. Subsequently, anticancer drug sensitivity tests were conducted on both 2D and 3D culture systems of colorectal adenocarcinoma cells. The results indicate that SCIOBIO III can mimic the extracellular matrix and serves as an ideal scaffold for constructing a 3D cell culture microenvironment. We successfully established a 3D culture model for colorectal adenocarcinoma cells and effectively screened anticancer drugs, which holds promise for advancing the development of personalized anticancer drug screening technologies.
近年来，结直肠癌的发病率逐年上升。在肿瘤治疗策略的研究过程中，基础研究成果向临床应用的转化常常受到现有肿瘤模型局限性的制约，迄今为止几乎没有取得突破。因此，我们的目标是探索利用自组装短肽（SAPs）构建用于结直肠腺癌细胞三维（3D）培养系统以进行抗癌药物筛选的可行性。通过对SAP SCIOBIO III的物理化学性质和生物相容性进行表征，我们证明它在离子触发下能迅速自组装成纳米纤维支架，支持3D细胞培养。随后，对结直肠腺癌细胞的二维和三维培养系统都进行了抗癌药物敏感性测试。结果表明，SCIOBIO III可以模拟细胞外基质，是构建3D细胞培养微环境的理想支架。我们成功建立了结直肠腺癌细胞的3D培养模型并有效筛选了抗癌药物，这为推进个性化抗癌药物筛选技术的发展带来了希望。
