The Key Laboratory of Cell Transplantation of Ministry of Health and Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
J Biomed Mater Res A. 2010 Sep 1;94(3):667-72. doi: 10.1002/jbm.a.32624.
Significant problems existing in the islet transplantation include a poor survival ability of the islet cells cultured under static conditions in vitro, decreased secretion function, and limited transplantation efficiency. In this study, we cocultured the three-dimensional (3D) self-assembling peptide nanofiber hydrogel scaffold with the islets from adult Wistar rats. The nanofiber scaffold constructed a 3D environment for the islets culture. The results of DTZ staining showed that the purity of the islets in the scaffold was >80%. The result of the fluorescent staining with AO-PI demonstrated that the viability of the islets in the 3D culture environment (within scaffold) was greater than those in the two-dimensional (2D) culture environment (without scaffold). The islets encapsulated in the 3D peptide nanofiber scaffold exhibited better secretion function. The insulin releasing index in the 3D group was remarkably higher than that in the 2D group. By scanning electron microscopy, it was observed that the 3D self-assembling peptide nanofiber hydrogel scaffold formed a nano scale fiber with a geometric form and the islets were encapsulated in this scaffold. Our research demonstrated that this nanofiber scaffold provided a favorable 3D environment for the islets to be cultured in vitro and then improve the secretion function and prolong the survival time of the islet in vitro.
胰岛细胞在体外静态培养条件下生存能力差、分泌功能下降以及移植效率有限。在这项研究中，我们将三维（3D）自组装肽纳米纤维水凝胶支架与成年 Wistar 大鼠的胰岛共培养。纳米纤维支架为胰岛培养构建了 3D 环境。DTZ 染色结果表明，支架内胰岛的纯度>80%。AO-PI 荧光染色结果表明，3D 培养环境（支架内）中胰岛的活力大于二维（2D）培养环境（无支架）。包封在 3D 肽纳米纤维支架中的胰岛表现出更好的分泌功能。3D 组的胰岛素释放指数显著高于 2D 组。通过扫描电子显微镜观察到，3D 自组装肽纳米纤维水凝胶支架形成了具有几何形状的纳米级纤维，胰岛被包裹在该支架中。我们的研究表明，这种纳米纤维支架为胰岛的体外培养提供了有利的 3D 环境，从而提高了胰岛的分泌功能并延长了其在体外的存活时间。
