Patterson T A, Kosh J W
College of Pharmacy, University of South Carolina, Columbia 29208.
Biol Mass Spectrom. 1992 Jun;21(6):299-304. doi: 10.1002/bms.1200210606.
A capillary gas chromatography/mass spectrometry (GC/MS) assay for the simultaneous quantitation of arecoline (ARE), acetylcholine (ACh), and choline (Ch) in biological tissue has been developed. The method utilizes hexadeuterated ARE and nonadeuterated ACh and Ch as internal standards. The compounds were ion-pair extracted from tissue using sodium tetraphenylboron in 3-heptanone. GC/MS analysis was achieved using capillary GC and electron impact mass spectrometry. Quantitation was accomplished using selected ion monitoring at m/z 140 and 146 for non-deuterated and deuterated arecoline respectively, and m/z 58 and 64 for non-deuterated and deuterated ACh and Ch respectively. The method easily detected 25 pmol of all three compounds taken through the assay, and was linear through 50 nmol.
已开发出一种用于同时定量生物组织中槟榔碱（ARE）、乙酰胆碱（ACh）和胆碱（Ch）的毛细管气相色谱/质谱（GC/MS）测定法。该方法使用十六氘代ARE以及十九氘代ACh和Ch作为内标。使用四苯硼钠在3 - 庚酮中从组织中进行离子对萃取这些化合物。通过毛细管气相色谱和电子轰击质谱实现GC/MS分析。分别使用m/z 140和146对非氘代和氘代槟榔碱进行选择离子监测，以及分别使用m/z 58和64对非氘代和氘代ACh和Ch进行选择离子监测来完成定量。该方法能够轻松检测出通过该测定法的所有三种化合物的25皮摩尔，并且在50纳摩尔范围内呈线性。
