从受感染个体外周血中分离、增殖和滴定1型人类免疫缺陷病毒。
Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals.
作者信息
Schuitemaker Hanneke, Kootstra Neeltje A
机构信息
Department of Clinical Vitro-Immunology, Sanquin Research at CLB, Academical Medical Center, Amsterdam, The Netherlands.
出版信息
Methods Mol Biol. 2005;304:17-24. doi: 10.1385/1-59259-907-9:017.
PMID:16061963
Abstract
HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided.
摘要
HIV-1可从外周血单个核细胞中分离出来，并且易于在原代细胞上进行体外培养。在此，我们描述了大量分离HIV-1准种的方法以及一种有限稀释病毒分离方案，通过该方案可获得单个共存克隆。此外，还提供了HIV-1的培养和滴定方法。
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