Petkov V, Brůcková M, Kopecká D, Syrůcek L, Vojtĕchovský K, Stanková M
Institut hygieny a epidemiologie, Praha.
Cesk Epidemiol Mikrobiol Imunol. 1990 Jun;39(3):129-33.
Using the method of co-cultivation with phytohaemagglutinin-stimulated lymphocytes from healthy donors, the author isolated the HIV-1 virus from peripheral mononuclear blood cells of three patients with the AIDS symptomatology and one patient with the ARC symptomatology. The presence of the virus in infected cells was proved by detection of the viral antigen p 24 in enzymatic immunoassays and in the immunofluorescence test. Three of the isolated strains were adapted to sensitive continual tissue cultures, where the isolates caused chronic infection of the cells associated with the development of a cytopathic effect. In the investigated patients no relationship was proved between viraemia and antigenaemia. The author discusses the importance of virus cultivation for laboratory diagnosis, epidemiology and research of HIV infection and AIDS.
作者采用与来自健康供体的经植物血凝素刺激的淋巴细胞共培养的方法，从3例有艾滋病症状的患者及1例有艾滋病相关综合征（ARC）症状的患者的外周血单个核细胞中分离出了HIV-1病毒。通过酶免疫测定法和免疫荧光试验检测病毒抗原p24，证实了感染细胞中存在该病毒。所分离出的3株病毒适应了敏感的连续组织培养，在这些培养中，分离株引起了与细胞病变效应发展相关的细胞慢性感染。在所研究的患者中，未证实病毒血症与抗原血症之间存在关联。作者讨论了病毒培养对于HIV感染和艾滋病的实验室诊断、流行病学及研究的重要性。
