Gueudin Marie, Damond Florence, Simon François
Unité de Virologie, GRAM EA 2636, CHU Charles Nicolle, Rouen, France.
Methods Mol Biol. 2005;304:215-20. doi: 10.1385/1-59259-907-9:215.
HIV-2 infection is confined mostly to West Africa. Seven HIV-2 subtypes have so far been described; only HIV-2 subtypes A and B are prevalent, the others being considered self-limiting infections at the epidemiological level. The main limitation for the HIV-2 DNA proviral quantification is the lack of HIV-2 DNA standard. We designed and tested a new HIV-2 primer couple that amplifies both the HIV-2 ROD strain and HIV-1 LAV/BRU strain. These HIV-2 primers were used to quantified an HIV-2 standard comparatively to a standard widely used in proviral DNA HIV-1 quantification, i.e., the 8E5 cell line transfected by a single defective integrated provirus of HIV-1 BRU/LAV by cell. The primers and probe used to quantify HIV-2 DNA are located in a long terminal repeat (LTR) region with low variability. These primers amplify both HIV-2 subtypes A and B. The relevance of the follow-up of the infected patients by the quantification of the proviral DNA HIV-2 is currently studied.
HIV-2感染主要局限于西非。迄今为止已描述了7种HIV-2亚型；只有HIV-2 A和B亚型流行，其他亚型在流行病学层面被认为是自限性感染。HIV-2 DNA前病毒定量的主要限制是缺乏HIV-2 DNA标准品。我们设计并测试了一对新的HIV-2引物，其能同时扩增HIV-2 ROD株和HIV-1 LAV/BRU株。这些HIV-2引物被用于与一种广泛用于前病毒DNA HIV-1定量的标准品（即通过细胞转染单个缺陷整合的HIV-1 BRU/LAV前病毒的8E5细胞系）相比较来定量一种HIV-2标准品。用于定量HIV-2 DNA的引物和探针位于变异性较低的长末端重复序列（LTR）区域。这些引物能扩增HIV-2 A和B两种亚型。目前正在研究通过定量HIV-2前病毒DNA对感染患者进行随访的相关性。
