Avanzini M A, Plebani A, Monafo V, Pasinetti G, Teani M, Colombo A, Mellander L, Carlsson B, Hanson L A, Ugazio A G
Department of Pediatrics, University of Pavia, Italy.
Acta Paediatr. 1992 Apr;81(4):296-301. doi: 10.1111/j.1651-2227.1992.tb12229.x.
In the present study salivary IgA, anti-Escherichia coli, anti-beta-lactoglobulin and anti-poliovirus type 1 IgA and IgM in serum and saliva were evaluated longitudinally in 13 breast-fed and 14 formula-fed infants over the first six months of life. Salivary IgA was quantified by electroimmunodiffusion; specific IgA and IgM antibodies were determined in serum and saliva by ELISA. Salivary IgA was significantly lower at age one month in breast-fed compared with formula-fed infants but in breast-fed infants salivary IgA increased with age and was significantly higher at six months than at one month. In both groups of infants, at the age of six months, salivary IgA levels were significantly lower than in adult controls. No significant differences in secretory anti-E. coli were observed between the two groups of infants. Salivary anti-poliovirus IgA and IgM antibodies increased transiently only to disappear in most babies at age six months, while anti-beta lactoglobulin IgA and IgM, present in saliva at all ages, showed a wide scatter. No important differences in specific serum IgA or IgM antibodies were observed either between the groups or at different times within the groups.
在本研究中，对13名母乳喂养婴儿和14名配方奶喂养婴儿在出生后的前六个月进行了纵向评估，检测了他们唾液中的免疫球蛋白A（IgA）、抗大肠杆菌、抗β-乳球蛋白以及血清和唾液中抗脊髓灰质炎病毒1型的IgA和IgM。唾液IgA通过电免疫扩散法定量；血清和唾液中的特异性IgA和IgM抗体通过酶联免疫吸附测定法（ELISA）测定。与配方奶喂养婴儿相比，母乳喂养婴儿在1个月大时唾液IgA显著较低，但母乳喂养婴儿的唾液IgA随年龄增长而增加，在6个月时显著高于1个月时。在两组婴儿中，6个月大时唾液IgA水平均显著低于成年对照组。两组婴儿之间未观察到分泌型抗大肠杆菌的显著差异。唾液抗脊髓灰质炎病毒IgA和IgM抗体仅短暂增加，在大多数婴儿6个月大时消失，而各年龄段唾液中均存在的抗β-乳球蛋白IgA和IgM则呈现出广泛的离散分布。两组之间或组内不同时间点的特异性血清IgA或IgM抗体均未观察到重要差异。
