Stephens S
Arch Dis Child. 1986 Mar;61(3):263-9. doi: 10.1136/adc.61.3.263.
Samples of saliva and nasal secretions were collected sequentially from 15 breast fed and 15 bottle fed infants on five occasions between 6 days and 9 months of age. Total immunoglobulin concentrations of G, M, and A classes, and class specific antibodies to tetanus toxoid and a pool of commensal strains of Escherichia coli were measured by solid phase radioimmunoassay and expressed per milligram of total protein. There were significant differences between feeding groups, which changed with age. Total IgM and IgA concentrations and IgA antibodies to E. coli were higher in the saliva and nasal secretions of breast fed infants at 6 days. There followed a rapid increase in IgM and IgA concentrations in secretions from all infants, and between 6 weeks and 9 months concentrations were higher in the saliva (but not in the nasal secretions) of the bottle fed group. There were no significant differences between the feeding groups for total IgG, specific G, M, and A antibodies to tetanus toxoid, and G and M antibodies to E. coli. These results suggest that breast feeding enhances secretory immunity in the early neonatal period only. By 6 weeks, local antigens are the main source of stimulation for production of immunoglobulin in the respiratory mucosa and thus may be obscuring any additional stimulation by growth factors in breast milk.
在15名母乳喂养婴儿和15名人工喂养婴儿6日龄至9月龄期间，分五次依次采集唾液和鼻分泌物样本。采用固相放射免疫分析法测定G、M和A类免疫球蛋白的总浓度，以及破伤风类毒素和一组大肠杆菌共生菌株的类特异性抗体，并以每毫克总蛋白表示。喂养组之间存在显著差异，且随年龄变化。6日龄时，母乳喂养婴儿的唾液和鼻分泌物中总IgM和IgA浓度以及抗大肠杆菌的IgA抗体较高。随后，所有婴儿分泌物中的IgM和IgA浓度迅速增加，6周龄至9月龄期间，人工喂养组婴儿的唾液（而非鼻分泌物）中浓度较高。母乳喂养组和人工喂养组在总IgG、破伤风类毒素特异性G、M和A抗体以及抗大肠杆菌的G和M抗体方面无显著差异。这些结果表明，母乳喂养仅在新生儿早期增强分泌性免疫。到6周龄时，局部抗原是呼吸道黏膜免疫球蛋白产生的主要刺激源，因此可能掩盖了母乳中生长因子的任何额外刺激。
