Mowrey-McKee M F, Monnat K, Sampson H J, Smith C M, Davies G A, Mandt L, Proskin H M
Personal Products Division, Bausch & Lomb, Rochester, NY.
CLAO J. 1992 Apr;18(2):87-91.
We cultured Soflens (polymacon) contact lenses to determine the number of microorganisms present following normal patient wear and handling just prior to disinfection. Total protein deposited was determined for the companion lens from each patient. A random population of 109 adapted soft contact lens patients participated in the study. Some patients participated more than once, resulting in a total of 196 lenses being cultured and 195 lenses analyzed for total protein. The left lens was cultured immediately. The right lens was extracted at 70 degrees C in sodium hydroxide, and the total protein in the extract determined using a modified Lowry protein assay. The mean protein deposition per lens was 3.4 micrograms (median 2 micrograms per lens; range less than 1 to 78 micrograms/lens). Microorganisms were cultured from 95% of the lenses. The mean bacterial count (in colony forming units per lens) was 2,482 (median: 123; range less than 3 to 150,000). Fungal contamination was found on 11% of the lenses at very low levels (3-9 yeast/lens and 3-18 mold/lens). Statistical analysis found no significant relationship between bacterial bioburden and any of the study parameters, including total protein, lens age, or subjective evaluation of lens cleanliness.
我们对Soflens（聚甲基丙烯酸甲酯）隐形眼镜进行培养，以确定在正常患者佩戴和消毒前处理后存在的微生物数量。测定了每位患者配套镜片上沉积的总蛋白量。随机抽取109名适配软性隐形眼镜的患者参与该研究。一些患者参与不止一次，总共培养了196片镜片，并对195片镜片进行了总蛋白分析。立即对左眼镜片进行培养。右眼镜片在70摄氏度的氢氧化钠中提取，提取物中的总蛋白使用改良的Lowry蛋白测定法测定。每片镜片的平均蛋白沉积量为3.4微克（中位数为每片镜片2微克；范围为每片镜片少于1至78微克）。95%的镜片培养出了微生物。平均细菌计数（每片镜片的菌落形成单位数）为2482（中位数：123；范围为每片镜片少于3至150,000）。在11%的镜片上发现了极低水平的真菌污染（每片镜片有3 - 9个酵母菌和3 - 18个霉菌）。统计分析发现细菌生物负荷与任何研究参数之间均无显著关系，这些参数包括总蛋白、镜片使用时长或镜片清洁度的主观评估。
