Hart D E, Reindel W, Proskin H M, Mowrey-McKee M F
Department of Ophthalmology, Harkness Eye Institute, Columbia University, New York, New York.
Optom Vis Sci. 1993 Mar;70(3):185-91. doi: 10.1097/00006324-199303000-00002.
Previous studies have demonstrated the presence of microorganisms on hydrogel contact lenses under various usage conditions. We conducted this study to quantify and identify viable bacteria and fungi associated with hydrogel contact lenses while on the eye. We removed the lenses from patients' eyes using aseptic techniques and cultured them to identify loosely adherent, as well as lens bound, microorganisms. Lenses were vortexed in a transfer medium (thereafter called the lens extract) and the lenses were then incubated in an agar sandwich separately from the transfer medium. We cultured 108 lenses (82 daily wear and 26 extended wear) from 49 patients. Bacteria were cultured from 38% (41) of the lenses; for 31 of these 41 lenses bacteria were isolated only from the lens extracts (made by vortexing lenses in a transfer medium), suggesting a transient association with the lenses. No fungi were isolated. Counts of less than 10 colony forming units (CFU)/lens were observed on 89% of the lenses. Staphylococcus epidermidis were the most frequently isolated bacteria. A statistically significant relation was observed between increased CFU/lens and increased lens age for extended wear lenses (p = 0.028).
以往研究已证实在各种使用条件下，水凝胶隐形眼镜上存在微生物。我们开展本研究以量化并鉴定与佩戴在眼上的水凝胶隐形眼镜相关的活细菌和真菌。我们采用无菌技术从患者眼中取出镜片并进行培养，以鉴定松散附着以及与镜片结合的微生物。将镜片在转移介质（此后称为镜片提取物）中涡旋处理，然后将镜片与转移介质分开在琼脂夹层中孵育。我们培养了来自49名患者的108片镜片（82片日戴型和26片长戴型）。38%（41片）的镜片培养出细菌；在这41片镜片中，有31片仅从镜片提取物（通过在转移介质中涡旋镜片制成）中分离出细菌，表明与镜片存在短暂关联。未分离出真菌。89%的镜片上观察到菌落形成单位（CFU）/镜片数少于10。表皮葡萄球菌是最常分离出的细菌。对于长戴型镜片，观察到CFU/镜片数增加与镜片使用时长增加之间存在统计学显著相关性（p = 0.028）。
