Chappuis P, Poupon J, Rousselet F
Laboratoire de Biochimie, Hôpital Lariboisière, Paris, France.
Clin Chim Acta. 1992 Mar 31;206(3):155-65. doi: 10.1016/0009-8981(92)90085-5.
A sequential method of measuring zinc, copper and aluminium in serum by inductively coupled plasma (ICP) spectrometry is described. It involves a 1/5 dilution of serum with a potassium chloride solution which enhances aluminium signal intensity and reduces variations between different matrix compositions. The method is as sensitive as atomic absorption for zinc (sensitivity: 0.11 mumol/l) and copper (sensitivity: 0.020 mumol/l) and can also be applied to monitor aluminium (sensitivity: 0.12 mumol/l) for patients receiving total nutrition therapy or hemodialysis. Its linearity extends at least to 200 mumol/l for copper and zinc and to 20 mumol/l for aluminium. The correlations with atomic absorption are satisfactory for the 3 parameters, as assessed by the correlation coefficients established for both methods. A reference interval was established with 34 sera of control subjects (19 men, 15 women) which showed an average zinc, copper and aluminium of 14.5 (S.D. 2.6), 17.3 (S.D. 2.1) and 0.32 (S.D. 0.12) mumol/l, respectively. This method does not require a simultaneous ICP spectrometer and can be performed with 1 ml of serum in a single tube, using a routine sequential ICP spectrometer.
本文描述了一种通过电感耦合等离子体（ICP）光谱法顺序测定血清中锌、铜和铝的方法。该方法包括用氯化钾溶液对血清进行1/5稀释，这可增强铝的信号强度并减少不同基质成分之间的差异。该方法对锌（灵敏度：0.11μmol/l）和铜（灵敏度：0.020μmol/l）的灵敏度与原子吸收法相当，也可用于监测接受全营养治疗或血液透析患者的铝（灵敏度：0.12μmol/l）。其线性范围至少对铜和锌延伸至200μmol/l，对铝延伸至20μmol/l。通过两种方法建立的相关系数评估，这三个参数与原子吸收法的相关性令人满意。用34份对照受试者血清（19名男性，15名女性）建立了参考区间，其锌、铜和铝的平均值分别为14.5（标准差2.6）、17.3（标准差2.1）和0.32（标准差0.12）μmol/l。该方法不需要同时配备ICP光谱仪，使用常规顺序ICP光谱仪，在单管中用1ml血清即可进行检测。
