Matsuyama S, Shiota K, Tachi C, Nishihara M, Takahashi M
Department of Veterinary Physiology, University of Tokyo, Japan.
Endocrinol Jpn. 1992 Feb;39(1):51-7. doi: 10.1507/endocrj1954.39.51.
We have reported that splenic macrophages play a role in the regulation of progestin secretion in rats. In this study, splenic macrophages were obtained from cycling rats at different estrous cycle stages and co-cultured with luteal cells from mid-pseudopregnant rats in the absence/presence of prolactin (PRL) or luteinizing hormone (LH). The effect of macrophages on the luteotropic action of PRL and LH was evaluated with 2 parameters, i.e. an increase in total progestin output (progesterone plus 20 alpha-hydroxyprgn-4-en-one [20 alpha-OHP]), and an increase in the progesterone to 20 alpha-OHP (P/20 alpha-OHP) secretion ratio. Splenic macrophages obtained from proestrous or metestrous rats enhanced the PRL action to increase the P/20 alpha-OHP secretion ratio, but those from estrous or diestrous donors did not. Only macrophages from proestrous donors enhanced the PRL action to increase the total progestin output. In contrast, the LH action increasing the P/20 alpha-OHP secretion ratio was enhanced by splenic macrophages regardless of the donors' estrous cycle stages. The LH action increasing the total progestin output was enhanced only by proestrous or metestrous macrophages. Therefore, if luteal cells are co-cultured with proestrous macrophages, the luteotropic actions of PRL and LH can be fully expressed. These results indicate that splenic macrophages directly act on luteal cells and enhance the luteotropic action of PRL and LH, and that this function of splenic macrophages is modified somehow according to the donors' estrous cycle stages.
我们曾报道脾脏巨噬细胞在大鼠孕激素分泌调节中发挥作用。在本研究中，从处于不同发情周期阶段的周期性大鼠获取脾脏巨噬细胞，并在有无催乳素（PRL）或促黄体生成素（LH）的情况下，与假孕中期大鼠的黄体细胞进行共培养。用两个参数评估巨噬细胞对PRL和LH促黄体作用的影响，即总孕激素输出量（孕酮加20α-羟基孕-4-烯-3-酮[20α-OHP]）的增加，以及孕酮与20α-OHP（P/20α-OHP）分泌比率的增加。从动情前期或动情后期大鼠获取的脾脏巨噬细胞增强了PRL增加P/20α-OHP分泌比率的作用，但来自发情期或动情间期供体的巨噬细胞则没有。只有来自动情前期供体的巨噬细胞增强了PRL增加总孕激素输出量的作用。相反，无论供体的发情周期阶段如何，脾脏巨噬细胞均增强了LH增加P/20α-OHP分泌比率的作用。只有动情前期或动情后期的巨噬细胞增强了LH增加总孕激素输出量的作用。因此，如果将黄体细胞与动情前期巨噬细胞共培养，PRL和LH的促黄体作用可得到充分表达。这些结果表明，脾脏巨噬细胞直接作用于黄体细胞并增强PRL和LH的促黄体作用，且脾脏巨噬细胞的这一功能会根据供体的发情周期阶段以某种方式发生改变。
