Yamanouchi K, Matsuyama S, Nishihara M, Shiota K, Tachi C, Takahashi M
Department of Veterinary Physiology, University of Tokyo, Japan.
Biol Reprod. 1992 Jun;46(6):1109-13. doi: 10.1095/biolreprod46.6.1109.
The effect of splenic macrophages on in vitro progestin secretion (the sum of the progesterone and 4-pregnen-20 alpha-ol-3-one concentrations in the medium) from mature rat granulosa cells was examined by means of co-culture techniques. When splenic macrophages (3.0 x 10(5) cells/ml) obtained from adult female rats on the evening of proestrus (1800 h) were added to granulosa cells (1.5 x 10(5) cells/ml) and co-cultured for 96 h in the absence of prolactin (PRL), progestin secretion from granulosa cells did not change. However, co-culture of granulosa cells with the macrophages in the presence of PRL (2 micrograms/ml) significantly enhanced progestin secretion after 48 h of culture. This stimulatory effect on progestin secretion was observed only when the number of macrophages added was more than twice the number of granulosa cells. On the other hand, splenic macrophages obtained on the evening of diestrus had no effect on progestin secretion from granulosa cells even in the presence of PRL. These results suggest that splenic macrophages can enhance PRL action so as to stimulate progestin secretion from granulosa cells and that this function of splenic macrophages varies during the estrous cycle.
通过共培养技术研究了脾巨噬细胞对成熟大鼠颗粒细胞体外孕激素分泌（培养基中孕酮和4-孕烯-20α-醇-3-酮浓度之和）的影响。当将从发情前期晚上（1800时）成年雌性大鼠获得的脾巨噬细胞（3.0×10⁵个细胞/毫升）加入到颗粒细胞（1.5×10⁵个细胞/毫升）中，并在无催乳素（PRL）的情况下共培养96小时时，颗粒细胞的孕激素分泌没有变化。然而，在PRL（2微克/毫升）存在的情况下，颗粒细胞与巨噬细胞共培养48小时后，显著增强了孕激素分泌。仅当添加的巨噬细胞数量超过颗粒细胞数量的两倍时，才观察到对孕激素分泌的这种刺激作用。另一方面，在动情后期晚上获得的脾巨噬细胞即使在有PRL的情况下对颗粒细胞的孕激素分泌也没有影响。这些结果表明，脾巨噬细胞可以增强PRL的作用，从而刺激颗粒细胞的孕激素分泌，并且脾巨噬细胞的这种功能在发情周期中有所变化。
