Baba M, Kobayashi T, Tamaki Y, Mishima H, Yagyu T, Morimoto H, Monden T, Shimano T, Tsuji Y, Murakami H
Department of Surgery II, Osaka University Medical School, Japan.
Hybridoma. 1992 Apr;11(2):107-19. doi: 10.1089/hyb.1992.11.107.
A human monoclonal antibody, BMMK-33G, was established by a fusion of human B-lymphoblastoid cells, HO-323, with lymphocytes of axillary lymph nodes obtained from a breast cancer patient. High-performance thin-layer chromatography (HPTLC)-immunostaining and enzyme-linked immunosorbent assay (ELISA) revealed that BMMK-33G was interestingly directed to enough sulfatide (Galactosylceramid-I2-sulfate), which is one of the sulfate ester containing glycolipids. By immunohistochemical staining, BMMK-33G intensely reacted to breast cancer, pancreatic cancer and gastric cancer. It also reacted to many normal human tissues including mammary glands, but these stainings were weaker than those for cancer. This report describes BMMK-33G, a human monoclonal antibody against sulfatide which may be very useful for studying not only tumor immunology but also autoimmune diseases.
一种人源单克隆抗体BMMK - 33G，是通过将人B淋巴母细胞系HO - 323与一名乳腺癌患者腋窝淋巴结的淋巴细胞融合而建立的。高效薄层色谱（HPTLC）免疫染色和酶联免疫吸附测定（ELISA）显示，BMMK - 33G有趣地靶向足够量的硫脂（半乳糖神经酰胺 - I2 - 硫酸盐），硫脂是含硫酸酯的糖脂之一。通过免疫组织化学染色，BMMK - 33G对乳腺癌、胰腺癌和胃癌有强烈反应。它对包括乳腺在内的许多正常人体组织也有反应，但这些染色比癌症组织的染色弱。本报告描述了BMMK - 33G，一种针对硫脂的人源单克隆抗体，它不仅可能对肿瘤免疫学研究非常有用，而且对自身免疫性疾病研究也很有用。
