• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
A cancer-reactive human monoclonal antibody derived from a colonic cancer patient treated with local immunotherapy.一种源自接受局部免疫治疗的结肠癌患者的癌症反应性人单克隆抗体。
Jpn J Cancer Res. 1993 Jan;84(1):75-82. doi: 10.1111/j.1349-7006.1993.tb02787.x.
2
Use of a local immunotherapy as an adjunctive tool for the generation of human monoclonal antibodies from regional lymph nodes of colonic cancer patients.使用局部免疫疗法作为从结肠癌患者区域淋巴结产生人单克隆抗体的辅助工具。
Jpn J Cancer Res. 1992 Jan;83(1):20-3. doi: 10.1111/j.1349-7006.1992.tb02346.x.
3
Augmentation of antitumor immunity in regional lymph nodes by local immunotherapy.通过局部免疫疗法增强区域淋巴结中的抗肿瘤免疫力。
Biotherapy. 1993;6(2):103-12. doi: 10.1007/BF01877423.
4
Isolation and fine-structure characterization of four monoclonal antibodies reactive with glycoconjugates containing terminal GlcNAc residues: application to aspects of lacto-series tumor antigen biosynthesis.
Arch Biochem Biophys. 1991 Jul;288(1):87-96. doi: 10.1016/0003-9861(91)90168-i.
5
A human monoclonal antibody derived from axillary lymph nodes of a breast cancer patient reactive to a sulfated glycolipid.一种源自乳腺癌患者腋窝淋巴结、对硫酸化糖脂有反应的人单克隆抗体。
Hybridoma. 1992 Apr;11(2):107-19. doi: 10.1089/hyb.1992.11.107.
6
Establishment and evaluation of cancer-specific human monoclonal antibody GAH for targeting chemotherapy using immunoliposomes.用于免疫脂质体靶向化疗的癌症特异性人单克隆抗体GAH的建立与评价
Hybrid Hybridomics. 2004 Apr;23(2):109-20. doi: 10.1089/153685904774129711.
7
Human IgG3 monoclonal antibody directed to an unbranched repeating type 2 chain (Gal beta 1----4GlcNAc beta 1----3Gal beta 1----4GlcNAc beta 1----3Gal beta 1----R) which is highly expressed in colonic and hepatocellular carcinoma.人IgG3单克隆抗体，其针对在结肠癌和肝细胞癌中高表达的无分支重复2型链（Galβ1----4GlcNAcβ1----3Galβ1----4GlcNAcβ1----3Galβ1----R）。
Cancer Res. 1989 Oct 15;49(20):5689-95.
8
Establishment of an anti-A human monoclonal antibody from a blood group A lung cancer patient: evidence for the occurrence of autoimmune response to difucosylated type-2 chain A.从一名A型血肺癌患者体内建立抗A人单克隆抗体：对二岩藻糖基化2型A链发生自身免疫反应的证据
Eur J Immunol. 1989 Dec;19(12):2197-203. doi: 10.1002/eji.1830191204.
9
A novel tumor-associated, developmentally regulated glycolipid antigen defined by monoclonal antibody ACFH-18.一种由单克隆抗体ACFH-18定义的新型肿瘤相关、发育调控糖脂抗原。
J Biol Chem. 1988 Sep 25;263(27):13942-51.
10
Localization of tumor-reactive lymph node lymphocytes in vivo using radiolabeled monoclonal antibody.使用放射性标记单克隆抗体在体内对肿瘤反应性淋巴结淋巴细胞进行定位
Cancer. 1994 Feb 1;73(3):580-9. doi: 10.1002/1097-0142(19940201)73:3<580::aid-cncr2820730314>3.0.co;2-b.

引用本文的文献

1
Analysis of cytotoxic activity of the CD4+ T lymphocytes generated by local immunotherapy.局部免疫疗法产生的CD4 + T淋巴细胞的细胞毒性活性分析。
Br J Cancer. 1996 Jan;73(1):110-6. doi: 10.1038/bjc.1996.20.

本文引用的文献

1
Detection of gangliosides that bind cholera toxin: direct binding of 125I-labeled toxin to thin-layer chromatograms.结合霍乱毒素的神经节苷脂的检测：125I标记毒素与薄层层析图的直接结合
Anal Biochem. 1980 Dec;109(2):399-402. doi: 10.1016/0003-2697(80)90667-3.
2
New globoseries glycosphingolipids in human teratocarcinoma reactive with the monoclonal antibody directed to a developmentally regulated antigen, stage-specific embryonic antigen 3.人畸胎癌中与针对发育调控抗原（阶段特异性胚胎抗原3）的单克隆抗体发生反应的新型globoseries糖鞘脂。
J Biol Chem. 1983 Jul 25;258(14):8934-42.
3
Human monoclonal antibody to ganglioside GD2-inhibited human melanoma xenograft.抗神经节苷脂GD2的人单克隆抗体抑制人黑色素瘤异种移植。
Eur J Cancer Clin Oncol. 1984 Aug;20(8):1053-9. doi: 10.1016/0277-5379(84)90108-1.
4
A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen SSEA-1.一系列与针对发育调控抗原SSEA-1的单克隆抗体发生反应的人红细胞糖鞘脂。
J Biol Chem. 1982 Dec 25;257(24):14865-74.
5
Generation of tumor cell-reactive human monoclonal antibodies using peripheral blood lymphocytes from actively immunized colorectal carcinoma patients.利用主动免疫的结直肠癌患者外周血淋巴细胞产生肿瘤细胞反应性人单克隆抗体。
Cancer Res. 1985 Aug;45(8):3951-61.
6
Human-human hybridomas for the study of anti-tumor immune response in patients with colorectal cancer.
Int J Cancer. 1986 May 15;37(5):683-8. doi: 10.1002/ijc.2910370507.
7
Structures of glycosphingolipids isolated from human embryonal carcinoma cells. The presence of mono- and disialosyl glycolipids with blood group type 1 sequence.从人胚胎癌细胞中分离出的糖鞘脂结构。具有血型1序列的单唾液酸和双唾液酸糖脂的存在。
J Biol Chem. 1986 Apr 15;261(11):5145-53.
8
HO-323, a human B-lymphoblastoid cell line useful for making human-human hybridomas.HO-323，一种可用于制备人-人杂交瘤的人B淋巴细胞系。
Cell Biol Int Rep. 1986 Feb;10(2):77-83. doi: 10.1016/0309-1651(86)90090-1.
9
Regression of cutaneous metastatic melanoma by intralesional injection with human monoclonal antibody to ganglioside GD2.通过病灶内注射抗神经节苷脂GD2人单克隆抗体使皮肤转移性黑色素瘤消退。
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8694-8. doi: 10.1073/pnas.83.22.8694.
10
Specificity analysis of human monoclonal antibodies reactive with cell surface and intracellular antigens.与细胞表面和细胞内抗原反应的人单克隆抗体的特异性分析
Proc Natl Acad Sci U S A. 1986 May;83(9):2959-63. doi: 10.1073/pnas.83.9.2959.

一种源自接受局部免疫治疗的结肠癌患者的癌症反应性人单克隆抗体。

A cancer-reactive human monoclonal antibody derived from a colonic cancer patient treated with local immunotherapy.

作者信息

Yagyu T, Monden T, Baba M, Tamaki Y, Takeda T, Kobayashi T, Shimano T, Tsuji Y, Matsushita H, Osawa H

机构信息

Second Department of Surgery, Osaka University Medical School.

出版信息

Jpn J Cancer Res. 1993 Jan;84(1):75-82. doi: 10.1111/j.1349-7006.1993.tb02787.x.

DOI:10.1111/j.1349-7006.1993.tb02787.x
PMID:8449830
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5919026/
Abstract

A human monoclonal antibody, YJ-37 (IgM) was generated through the fusion of human B lymphoblastoid cell line HO-323 with the regional lymph node lymphocytes from a colonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ-37 selectively reacted with colonic cancer (7/19), gastric cancer (3/6), endometrial cancer (1/2) and colonic adenoma (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ-37 bound to tumor cell surfaces. Furthermore, the chemical structure of the antigen defined by YJ-37 was analyzed by means of thin-layer chromatography immunostaining and ELISA. The results indicated that YJ-37 reacted with sialylated lacto-series carbohydrate chains, which have been reported to accumulate in cancer cells.

摘要

通过将人B淋巴母细胞系HO-323与一名接受局部免疫治疗的结肠癌患者的区域淋巴结淋巴细胞融合，产生了一种人单克隆抗体YJ-37（IgM）。该抗体经纯化后与生物素偶联，随后进行直接免疫组织化学染色。结果显示，YJ-37与结肠癌（7/19）、胃癌（3/6）、子宫内膜癌（1/2）和结肠腺瘤（7/13）有选择性反应，但与正常上皮无反应。膜免疫荧光和流式细胞术分析也表明YJ-37与肿瘤细胞表面结合。此外，通过薄层色谱免疫染色和酶联免疫吸附测定法分析了YJ-37所定义抗原的化学结构。结果表明，YJ-37与唾液酸化乳糖系列碳水化合物链发生反应，据报道这些碳水化合物链在癌细胞中积累。