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一种源自接受局部免疫治疗的结肠癌患者的癌症反应性人单克隆抗体。

A cancer-reactive human monoclonal antibody derived from a colonic cancer patient treated with local immunotherapy.

作者信息

Yagyu T, Monden T, Baba M, Tamaki Y, Takeda T, Kobayashi T, Shimano T, Tsuji Y, Matsushita H, Osawa H

机构信息

Second Department of Surgery, Osaka University Medical School.

出版信息

Jpn J Cancer Res. 1993 Jan;84(1):75-82. doi: 10.1111/j.1349-7006.1993.tb02787.x.

DOI:10.1111/j.1349-7006.1993.tb02787.x
PMID:8449830
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5919026/
Abstract

A human monoclonal antibody, YJ-37 (IgM) was generated through the fusion of human B lymphoblastoid cell line HO-323 with the regional lymph node lymphocytes from a colonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ-37 selectively reacted with colonic cancer (7/19), gastric cancer (3/6), endometrial cancer (1/2) and colonic adenoma (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ-37 bound to tumor cell surfaces. Furthermore, the chemical structure of the antigen defined by YJ-37 was analyzed by means of thin-layer chromatography immunostaining and ELISA. The results indicated that YJ-37 reacted with sialylated lacto-series carbohydrate chains, which have been reported to accumulate in cancer cells.

摘要

通过将人B淋巴母细胞系HO-323与一名接受局部免疫治疗的结肠癌患者的区域淋巴结淋巴细胞融合，产生了一种人单克隆抗体YJ-37（IgM）。该抗体经纯化后与生物素偶联，随后进行直接免疫组织化学染色。结果显示，YJ-37与结肠癌（7/19）、胃癌（3/6）、子宫内膜癌（1/2）和结肠腺瘤（7/13）有选择性反应，但与正常上皮无反应。膜免疫荧光和流式细胞术分析也表明YJ-37与肿瘤细胞表面结合。此外，通过薄层色谱免疫染色和酶联免疫吸附测定法分析了YJ-37所定义抗原的化学结构。结果表明，YJ-37与唾液酸化乳糖系列碳水化合物链发生反应，据报道这些碳水化合物链在癌细胞中积累。

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Detection of gangliosides that bind cholera toxin: direct binding of 125I-labeled toxin to thin-layer chromatograms.结合霍乱毒素的神经节苷脂的检测：125I标记毒素与薄层层析图的直接结合
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HO-323, a human B-lymphoblastoid cell line useful for making human-human hybridomas.HO-323，一种可用于制备人-人杂交瘤的人B淋巴细胞系。
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