Iwata H, Takagi T, Amemiya H, Shimizu H, Yamashita K, Kobayashi K, Akutsu T
National Cardiovascular Center Research Institute, Osaka, Japan.
J Biomed Mater Res. 1992 Jul;26(7):967-77. doi: 10.1002/jbm.820260711.
Islets were encapsulated into 5% concentration agarose microbeads. The effect of microencapsulation on islet allograft survivals was determined using a streptozotocin-induced diabetic (STZ) mouse and a nonobese diabetic (NOD) mouse as recipients. All five STZ BALB/c mice receiving microencapsulated islets (C57BL/6) maintained normoglycemia indefinitely. When NOD mice were used as recipients of the bioartificial pancreas, four of five grafts (islets from C3H/He) functioned for more than 80 d. Two of five NOD mice maintained normoglycemia until animals were sacrificed at 102 and 192 postoperative d. Microbeads made of commercially available agarose can effectively prolong alloislets functioning in the STZ-diabetic mouse and even in the NOD mouse (animal model of human type I diabetes) without the use of any immunosuppressive drug.
胰岛被包封在5%浓度的琼脂糖微珠中。使用链脲佐菌素诱导的糖尿病（STZ）小鼠和非肥胖糖尿病（NOD）小鼠作为受体，确定微囊化对胰岛同种异体移植存活的影响。所有五只接受微囊化胰岛（C57BL/6）的STZ BALB/c小鼠均无限期维持正常血糖水平。当将NOD小鼠用作生物人工胰腺的受体时，五分之四的移植物（来自C3H/He的胰岛）功能超过80天。五只NOD小鼠中有两只维持正常血糖水平，直到在术后第102天和192天处死动物。由市售琼脂糖制成的微珠可以有效延长同种胰岛在STZ糖尿病小鼠甚至NOD小鼠（人类I型糖尿病动物模型）中的功能，而无需使用任何免疫抑制药物。
