使用自切割弹性蛋白样多肽标签的简单生物分离方法。
Simple bioseparations using self-cleaving elastin-like polypeptide tags.
作者信息
Banki Mahmoud Reza, Feng Liang, Wood David W
机构信息
Department of Chemical Engineering, A213 E-QUAD, Princeton University, Princeton, New Jersey 08544, USA.
出版信息
Nat Methods. 2005 Sep;2(9):659-61. doi: 10.1038/nmeth787.
DOI:10.1038/nmeth787
PMID:16074986
Abstract
We introduce a new method for the purification of recombinant proteins expressed in Escherichia coli using self-cleaving elastin-like polypeptide (ELP) fusion tags without the need for affinity chromatography or proteolytic tag removal. Using this method we obtained high purity, activity and reasonable yields for ten diverse target proteins.
摘要
我们介绍了一种新方法，用于纯化在大肠杆菌中表达的重组蛋白，该方法使用可自我切割的弹性蛋白样多肽（ELP）融合标签，无需亲和色谱法或去除蛋白水解标签。使用这种方法，我们获得了十种不同靶蛋白的高纯度、高活性和合理产量。
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