Qiusheng Zheng, Yuntao Zhang, Rongliang Zheng, Dean Guo, Changling Li
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Pharmazie. 2005 Jul;60(7):539-43.
Extensive but fragmentary studies have shown: (i) heroin, morphine and opiates are able to induce reactive oxygen species (ROS) formation in several cells, (ii) they decrease the antioxidant defense system including enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and antioxidants, glutathione (GSH), Se, and vitamins. This study is to investigate the oxidative damage to DNA, proteins, and lipids in brain of mice administered heroin via intraperitoneal injection, and the effects of verbascoside and luteolin on this damage. All the indices of oxidative damage, such as 8-hydroxy-2'-deoxyguanosine (8-OHdG), protein carbonyl group and malondialdehyde (MDA) contents increased significantly compared to those of controls in the brains of heroin-administered mice, while the indices related to the in vivo antioxidative capacity, such as the ratio of GSH and oxidized glutathione (GSSG), and activities of SOD, CAT and GPx in the brain, and total antioxidant capacity (TAC) in serum significantly decreased. When heroin-dependent mice were treated with verbascoside or luteolin, oxidative stress status was limited.
（i）海洛因、吗啡和阿片类药物能够在多种细胞中诱导活性氧（ROS）的形成，（ii）它们会降低抗氧化防御系统，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）等酶以及谷胱甘肽（GSH）、硒和维生素等抗氧化剂。本研究旨在探讨腹腔注射海洛因的小鼠脑内DNA、蛋白质和脂质的氧化损伤，以及毛蕊花糖苷和木犀草素对这种损伤的影响。与对照组相比，海洛因给药小鼠脑内所有氧化损伤指标，如8-羟基-2'-脱氧鸟苷（8-OHdG）、蛋白质羰基和丙二醛（MDA）含量均显著增加，而与体内抗氧化能力相关的指标，如脑内GSH与氧化型谷胱甘肽（GSSG）的比值、SOD、CAT和GPx的活性以及血清中的总抗氧化能力（TAC）则显著降低。当对海洛因依赖小鼠用毛蕊花糖苷或木犀草素进行治疗时，氧化应激状态受到限制。
