Bazoti Fotini N, Gikas Evangelos, Puel Caroline, Coxam Véronique, Tsarbopoulos Anthony
Department of Pharmacy, University of Patras, Panepistimiopolis, Rio 265 04, Greece.
J Agric Food Chem. 2005 Aug 10;53(16):6213-21. doi: 10.1021/jf050851w.
A novel gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed, using an ion trap mass spectrometer, for the simultaneous determination of olive oil bioactive components, elenolic acid, hydroxytyrosol, and tyrosol, in rat urine. Samples were analyzed by GC-MS/MS prior to and after enzymatic treatment. A solid phase extraction sample pretreatment step with greater than 80% analytical recoveries for all compounds was performed followed by a derivatization reaction prior to GC-MS/MS analysis. The calibration curves were linear for all compounds studied for a dynamic range between 1 and 500 ng. The limit of detection was in the mid picogram level for tyrosol and elenolic acid (300 pg) and in the low picogram level for hydroxytyrosol (2.5 pg). The method was applied to the analysis of rat urine samples after sustained oral intake of oleuropein or extra virgin olive oil as a diet supplement.
开发了一种使用离子阱质谱仪的新型气相色谱 - 串联质谱（GC-MS/MS）方法，用于同时测定大鼠尿液中的橄榄油生物活性成分，即橄榄苦苷酸、羟基酪醇和酪醇。在酶处理前后，通过GC-MS/MS对样品进行分析。进行了固相萃取样品预处理步骤，所有化合物的分析回收率均大于80%，然后在GC-MS/MS分析之前进行衍生化反应。在所研究的所有化合物中，校准曲线在1至500 ng的动态范围内呈线性。酪醇和橄榄苦苷酸的检测限处于中皮克水平（300 pg），羟基酪醇的检测限处于低皮克水平（2.5 pg）。该方法应用于大鼠持续口服橄榄苦苷或特级初榨橄榄油作为膳食补充剂后的尿液样本分析。
