使用设计的多胺衍生物在巨型DNA上特异性形成串珠状结构。

Specific formation of beads-on-a-chain structures on giant DNA using a designed polyamine derivative.

作者信息

Chen Ning, Zinchenko Anatoly A, Murata Shizuaki, Yoshikawa Kenichi

机构信息

Graduate School of Environmental Studies, Nagoya University, Chikusa, Nagoya 464-8601 Japan.

出版信息

J Am Chem Soc. 2005 Aug 10;127(31):10910-6. doi: 10.1021/ja042509q.

DOI:10.1021/ja042509q

PMID:16076197

Abstract

Fluorescence microscopy was used to study the folding transition of giant DNAs, T4 DNA (ca. 166 kbp), and lambda DNA (ca. 48 kbp), which proceeds through intermediates with intramolecular segregation induced by pteridine-polyamine conjugates, i.e., 2-amino-6,7-dimethyl-4-(4,9,13-triazatridecylamino)pteridine and -4-(3-(aminopropyl)amino)pteridine. According to the results of DNA denaturation, UV and fluorescent spectroscopy, and transmission electron microscopic observations, it became clear that DNA folding induced by the polyamine derivative is not a continuous shrinking process but a combination of discontinuous processes.

摘要

利用荧光显微镜研究了巨型DNA（T4 DNA，约166 kbp）和λ DNA（约48 kbp）的折叠转变，其通过由蝶啶 - 多胺缀合物诱导的分子内分离中间体进行，即2 - 氨基 - 6,7 - 二甲基 - 4 -（4,9,13 - 三氮杂十三烷基氨基）蝶啶和 - 4 -（3 -（氨丙基）氨基）蝶啶。根据DNA变性、紫外和荧光光谱以及透射电子显微镜观察结果，很明显，多胺衍生物诱导的DNA折叠不是一个连续的收缩过程，而是不连续过程的组合。