控制单个DNA分子上的链内分离。

Controlling the intrachain segregation on a single DNA molecule.

作者信息

Zinchenko Anatoly A, Sergeyev Vladimir G, Murata Shizuaki, Yoshikawa Kenichi

机构信息

Graduate School of Environmental Studies and CREST, JST (Japan Science and Technology), c/o School of Informatics and Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.

出版信息

J Am Chem Soc. 2003 Apr 16;125(15):4414-5. doi: 10.1021/ja028804x.

DOI:10.1021/ja028804x

PMID:12683799

Abstract

Intrachain segregation in single DNA molecules induced by quaternary ammonium dications was studied. By means of fluorescent and electron microscopy, it was found that variations in the chemical structure of condensing agents provide one with the opportunity to control the average amount of intrachain segregation centers on the DNA single chain. The manner of interaction between the diammonium molecules was considered to be the key factor for controlling the morphology of the partially collapsed DNA molecules.

摘要

研究了由季铵二价阳离子诱导的单个DNA分子内链分离。通过荧光显微镜和电子显微镜发现，凝聚剂化学结构的变化为控制DNA单链上链内分离中心的平均数量提供了机会。二铵分子之间的相互作用方式被认为是控制部分折叠的DNA分子形态的关键因素。