Ohtani Maki, Miyadai Toshiaki, Hiroishi Shingo
Research Center for Marine Bioresources, Faculty of Biotechnology, Fukui Prefectural University, 49-8-2 Katsumi, Obama, Fukui 917-0116, Japan.
Mol Immunol. 2006 Mar;43(7):1047-53. doi: 10.1016/j.molimm.2005.06.036. Epub 2005 Aug 1.
B-cell lymphoma-6 (BCL-6) is a transcriptional repressor that prevents the terminal differentiation of mature B-cells to plasma cells, and is essential for germinal center formation in the primary lymphoid organs of mammals. In this study, we identified the BCL-6 gene in torafugu (Takifugu rubripes) using the torafugu genome database, and analyzed the expression of BCL-6 mRNA in various tissues of torafugu, using RT-PCR. The BCL-6 gene consisted of eight exons and seven introns spanning a genome of ca. 3.3 kb. BCL-6 mRNA contained a 2112 bp open reading frame encoding 703 amino acids, with a predicted protein size of 78.8 kDa. The predicted torafugu BCL-6 primary structure contains two conserved specific motifs, the BTB/POZ domain at the N-terminus and the sixC2H2-type zinc finger motifs at the C-terminal region. The homology of torafugu BCL-6 to those of zebrafish (Danio rerio), Xenopus laevis, mouse (Mus musculus) and human (Homo sapiens) is 76, 59, 60 and 60%, respectively. RT-PCR analysis revealed that BCL-6 mRNA is highly expressed in pronephros, thymus, intestine, ovary, brain, nasal cavity and muscle. These results imply that torafugu BCL-6 is involved in regulation of B-cell differentiation in torafugu.
B细胞淋巴瘤-6（BCL-6）是一种转录抑制因子，可阻止成熟B细胞向浆细胞的终末分化，对哺乳动物初级淋巴器官生发中心的形成至关重要。在本研究中，我们利用红鳍东方鲀（Takifugu rubripes）基因组数据库鉴定了红鳍东方鲀中的BCL-6基因，并采用逆转录聚合酶链反应（RT-PCR）分析了BCL-6 mRNA在红鳍东方鲀各组织中的表达情况。BCL-6基因由8个外显子和7个内含子组成，跨越约3.3 kb的基因组。BCL-6 mRNA包含一个2112 bp的开放阅读框，编码703个氨基酸，预测蛋白质大小为78.8 kDa。预测的红鳍东方鲀BCL-6一级结构包含两个保守的特定基序，N端的BTB/POZ结构域和C端区域的六个C2H2型锌指基序。红鳍东方鲀BCL-6与斑马鱼（Danio rerio）、非洲爪蟾（Xenopus laevis）、小鼠（Mus musculus）和人类（Homo sapiens）的同源性分别为76%、59%、60%和60%。RT-PCR分析显示，BCL-6 mRNA在中肾、胸腺、肠、卵巢、脑、鼻腔和肌肉中高表达。这些结果表明，红鳍东方鲀BCL-6参与了红鳍东方鲀B细胞分化的调节。
