Schafer R, Portnoy D A, Brassell S A, Paterson Y
Department of Microbiology, University of Pennsylvania, Philadelphia 19104.
J Immunol. 1992 Jul 1;149(1):53-9.
A recombinant strain of Listeria monocytogenes that stably and constitutively expresses Escherichia coli beta-galactosidase was used as a live vaccine vector. BALB/c mice were immunized orally or parenterally with the recombinant L. monocytogenes, and their cellular and humoral immune responses to beta-galactosidase were measured. Spleen cells taken 1 week after oral inoculation or 5 weeks after oral or parenteral inoculation (with a boost at 4 weeks) showed beta-galactosidase-specific CTL responses. The CTL line derived from mice immunized i.p. was also shown to be class I restricted and Thy-1.2+, CD8+, and TCR alpha beta+. All mice immunized with the recombinant L. monocytogenes had positive delayed-type hypersensitivity responses to heat-killed L. monocytogenes, but only 15% had a positive delayed-type hypersensitivity reaction to beta-galactosidase. Individual serum samples from mice immunized i.p. or i.v. were tested for antibody to beta-galactosidase. Approximately 11% had low positive titers for beta-galactosidase antibodies. These results demonstrate that both oral and parenteral immunization with recombinant L. monocytogenes results in a cellular immune response to the foreign protein, which is primarily a specific CD8+ CTL response.
一种稳定且组成性表达大肠杆菌β-半乳糖苷酶的单核细胞增生李斯特菌重组菌株被用作活疫苗载体。用重组单核细胞增生李斯特菌对BALB/c小鼠进行口服或非肠道免疫，并检测它们对β-半乳糖苷酶的细胞免疫和体液免疫反应。口服接种1周后或口服或非肠道接种（4周时加强免疫）5周后采集的脾细胞显示出β-半乳糖苷酶特异性CTL反应。源自腹腔注射免疫小鼠的CTL系也显示为I类限制性，Thy-1.2+、CD8+和TCRαβ+。所有用重组单核细胞增生李斯特菌免疫的小鼠对热灭活的单核细胞增生李斯特菌均有阳性迟发型超敏反应，但只有15%对β-半乳糖苷酶有阳性迟发型超敏反应。对腹腔注射或静脉注射免疫小鼠的个体血清样本检测β-半乳糖苷酶抗体。约11%的小鼠β-半乳糖苷酶抗体滴度呈低阳性。这些结果表明，用重组单核细胞增生李斯特菌进行口服和非肠道免疫均可导致对异源蛋白的细胞免疫反应，主要是特异性CD8+CTL反应。
