Wnt信号通路在秀丽隐杆线虫早期胚胎中驱动WRM-1/β-连环蛋白的不对称性。
Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos.
作者信息
Nakamura Kuniaki, Kim Soyoung, Ishidate Takao, Bei Yanxia, Pang Kaming, Shirayama Masaki, Trzepacz Chris, Brownell Daniel R, Mello Craig C
机构信息
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
出版信息
Genes Dev. 2005 Aug 1;19(15):1749-54. doi: 10.1101/gad.1323705.
Abstract
beta-Catenin regulates cell adhesion and cellular differentiation during development, and misregulation of beta-catenin contributes to numerous forms of cancer in humans. Here we describe Caenorhabditis elegans conditional alleles of mom-2/Wnt, mom-4/Tak1, and wrm-1/beta-catenin. We use these reagents to examine the regulation of WRM-1/beta-catenin during a Wnt-signaling-induced asymmetric cell division. While WRM-1 protein initially accumulates in the nuclei of all cells, signaling promotes the retention of WRM-1 in nuclei of responding cells. We show that both PRY-1/Axin and the nuclear exportin homolog IMB-4/CRM-1 antagonize signaling. These findings reveal how Wnt signals direct the asymmetric localization of beta-catenin during polarized cell division.
摘要
β-连环蛋白在发育过程中调节细胞黏附和细胞分化，β-连环蛋白的调控异常会导致人类多种癌症。在此，我们描述了秀丽隐杆线虫中mom-2/Wnt、mom-4/Tak1和wrm-1/β-连环蛋白的条件等位基因。我们使用这些试剂来研究Wnt信号诱导的不对称细胞分裂过程中WRM-1/β-连环蛋白的调控。虽然WRM-1蛋白最初在所有细胞的细胞核中积累，但信号传导促进了WRM-1在响应细胞的细胞核中的保留。我们表明，PRY-1/Axin和核输出蛋白同源物IMB-4/CRM-1都拮抗信号传导。这些发现揭示了Wnt信号在极化细胞分裂过程中如何指导β-连环蛋白的不对称定位。
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