Takeshita Hisako, Sawa Hitoshi
Laboratory for Cell Fate Decision, Riken, Center for Developmental Biology, Kobe 650-0047, Japan.
Genes Dev. 2005 Aug 1;19(15):1743-8. doi: 10.1101/gad.1322805.
beta-Catenin can promote adhesion at the cell cortex and mediate Wnt signaling in the nucleus. We show that, in Caenorhabditis elegans, both WRM-1/beta-catenin and LIT-1 kinase localize to the anterior cell cortex during asymmetric cell division but to the nucleus of the posterior daughter afterward. Both the cortical and nuclear localizations are regulated by Wnts and are apparently coupled. We also found that the daughters show different nuclear export rates for LIT-1. Our results indicate that Wnt signals release cortical WRM-1 from the posterior cortex to generate cortical asymmetry that may control WRM-1 asymmetric nuclear localization by regulating cell polarity.
β-连环蛋白可促进细胞皮层的黏附并在细胞核中介导Wnt信号传导。我们发现，在秀丽隐杆线虫中，WRM-1/β-连环蛋白和LIT-1激酶在不对称细胞分裂期间定位于前细胞皮层，但随后定位于后子代的细胞核。皮层和细胞核定位均受Wnt调控，且明显相互关联。我们还发现子代对LIT-1表现出不同的核输出率。我们的结果表明，Wnt信号从后皮层释放皮层WRM-1以产生皮层不对称性，这可能通过调节细胞极性来控制WRM-1的不对称核定位。
