枯草芽孢杆菌孢子内膜上SpoVAD的定位
Localization of SpoVAD to the inner membrane of spores of Bacillus subtilis.
作者信息
Vepachedu Venkata Ramana, Setlow Peter
机构信息
Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA.
出版信息
J Bacteriol. 2005 Aug;187(16):5677-82. doi: 10.1128/JB.187.16.5677-5682.2005.
Abstract
The products of the hexacistronic spoVA operon of Bacillus subtilis may be involved in the transport of dipicolinic acid into the forespore during sporulation and its release during spore germination. The major hydrophilic coding region of B. subtilis spoVAD was cloned, the protein was expressed in Escherichia coli as a His tag fusion protein, and a rabbit antiserum was raised against the purified protein. Western blot analyses of fractions from B. subtilis spores showed that SpoVAD is an integral inner membrane protein present at levels >50-fold higher than those of the spore's nutrient germinant receptors that are also present in the inner membrane. SpoVAD also persisted in outgrowing spores.
摘要
枯草芽孢杆菌六顺反子spoVA操纵子的产物可能参与了芽孢形成过程中二吡啶羧酸向芽孢前体的转运以及芽孢萌发过程中其释放。克隆了枯草芽孢杆菌spoVAD的主要亲水性编码区，该蛋白在大肠杆菌中作为His标签融合蛋白表达，并制备了针对纯化蛋白的兔抗血清。对枯草芽孢杆菌芽孢各组分的蛋白质免疫印迹分析表明，SpoVAD是一种整合内膜蛋白，其含量比同样存在于内膜中的芽孢营养萌发受体高50倍以上。SpoVAD也存在于正在生长的芽孢中。
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