Cuñé Jordi, Cullen Paul, Mazon Gerard, Campoy Susana, Adler Ben, Barbe Jordi
Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
J Bacteriol. 2005 Aug;187(16):5841-5. doi: 10.1128/JB.187.16.5841-5845.2005.
Footprinting and mutagenesis experiments demonstrated that Leptospira interrogans LexA binds the palindrome TTTGN(5)CAAA found in the recA promoter but not in the lexA promoter. In silico analysis revealed that none of the other canonical SOS genes is under direct control of LexA, making the leptospiral lexA gene the first described which is not autoregulated.
足迹法和诱变实验表明,问号钩端螺旋体LexA结合recA启动子中发现的回文序列TTTGN(5)CAAA,但不结合lexA启动子中的该序列。计算机分析显示,其他典型的SOS基因均不受LexA的直接调控,这使得钩端螺旋体lexA基因成为首个被描述的非自我调节的此类基因。