Erill Ivan, Jara Mónica, Salvador Noelia, Escribano Marcos, Campoy Susana, Barbé Jordi
Biomedical Applications Group, Centro Nacional de Microelectrónica, 08193 Bellaterra, Spain.
Nucleic Acids Res. 2004 Dec 16;32(22):6617-26. doi: 10.1093/nar/gkh996. Print 2004.
The LexA regulon encompasses an ensemble of genes involved in preserving cell viability under massive DNA damage and is present in most bacterial phyla. Up to date, however, the scope of this network had only been assessed in the Gamma Proteobacteria. Here, we report the structure of the LexA regulon in the Alpha Proteobacteria, using a combined approach that makes use of in vitro and in vivo techniques to assist and validate the comparative genomics in silico methodology. This leads to the first experimentally validated description of the LexA regulon in the Alpha Proteobacteria, and comparison of regulon core structures in both classes suggests that a least common multiple set of genes (recA, ssb, uvrA and ruvCAB) might be a defining property of the Proteobacteria LexA network.
LexA调控子包含一组在大量DNA损伤情况下参与维持细胞活力的基因,并且存在于大多数细菌门类中。然而,截至目前,该网络的范围仅在γ-变形菌纲中得到评估。在此,我们报道了α-变形菌纲中LexA调控子的结构,采用了一种结合体外和体内技术的方法,以辅助和验证基于计算机的比较基因组学方法。这导致了对α-变形菌纲中LexA调控子的首次实验验证描述,并且对这两个纲中调控子核心结构的比较表明,一组最少共同的基因(recA、ssb、uvrA和ruvCAB)可能是变形菌纲LexA网络的一个决定性特征。