Furuta Chie, Li ChunMei, Taneda Shinji, Suzuki Akira K, Kamata Kazuyuki, Watanabe Gen, Taya Kazuyoshi
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu Univ, Gifu, Japan.
Endocrine. 2005 Jun;27(1):33-6. doi: 10.1385/ENDO:27:1:033.
We recently isolated 3-methyl-4-nitrophenol (4-nitro-m-cresol; PNMC) and 4-nitro-3-phenylphenol (PNMPP) from diesel exhaust particles (DEP) and identified them as vasodilators and xenoestrogens. The estrogenic activity of PNMC and PNMPP was further examined by using immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in uterine luminal epithelium of ovariectomized 25-d-old immature female rats injected with PNMC and PNMPP subcutaneously for 2 d. Significant increases were observed in uterine luminal epithelium in PCNA positive cells of animals receiving 10 and 100 mg/kg PNMC and 0.1 mg/kg PNMPP compared with controls. These results clearly show the estrogenic activity of PNMC and PNMPP by cell proliferation on the uterine luminal epithelium.
我们最近从柴油废气颗粒（DEP）中分离出3-甲基-4-硝基苯酚（4-硝基间甲酚；PNMC）和4-硝基-3-苯基苯酚（PNMPP），并将它们鉴定为血管舒张剂和外源性雌激素。通过对皮下注射PNMC和PNMPP 2天的25日龄未成熟雌性去卵巢大鼠子宫腔上皮细胞增殖细胞核抗原（PCNA）进行免疫组织化学染色，进一步检测了PNMC和PNMPP的雌激素活性。与对照组相比，接受10和100 mg/kg PNMC以及0.1 mg/kg PNMPP的动物子宫腔上皮细胞中PCNA阳性细胞显著增加。这些结果清楚地表明，PNMC和PNMPP通过子宫腔上皮细胞增殖具有雌激素活性。
