Jagadish Nirmala, Rana Ritu, Mishra Deepshikha, Kumar Manoj, Suri Anil
Genes and Proteins Laboratory, National Institute of Immunology, New Delhi, India.
Keio J Med. 2005 Jun;54(2):66-71. doi: 10.2302/kjm.54.66.
Previously, we cloned and sequenced a novel human sperm associated antigen 9 (SPAG9). Northern blot analysis and RNA in situ hybridization experiments revealed testis- and stage-specific expression of SPAG9 mRNA, mainly confined to round spermatid suggesting haploid germ cell expression Studies on the human and non-human primates (macaque and baboon) have shown a homology of 84.9% and 90.6% at amino acid level and 94% and 96.8% at DNA level, respectively. The presence of high level of homology at amino acid and DNA level indicates that SPAG9 is conserved in human, baboon and macaque sharing common function and common origin in the biological past. In addition, SPAG9 protein revealed structural homology with c-Jun NH2-terminal kinase (JNK) interacting protein (JIP). The amino acid sequence analysis of SPAG9 predicted coiled coil, leucine zipper and transmembrane domain, speculating the involvement of SPAG9 mediated signal transduction pathways in reproductive processes.
此前，我们克隆并测序了一种新型人类精子相关抗原9（SPAG9）。Northern印迹分析和RNA原位杂交实验显示，SPAG9 mRNA在睾丸中呈阶段特异性表达，主要局限于圆形精子细胞，提示单倍体生殖细胞表达。对人类和非人类灵长类动物（猕猴和狒狒）的研究表明，在氨基酸水平上的同源性分别为84.9%和90.6%，在DNA水平上分别为94%和96.8%。氨基酸和DNA水平上的高度同源性表明，SPAG9在人类、狒狒和猕猴中保守，在生物进化史上具有共同的功能和起源。此外，SPAG9蛋白与c-Jun氨基末端激酶（JNK）相互作用蛋白（JIP）显示出结构同源性。SPAG9的氨基酸序列分析预测其具有卷曲螺旋、亮氨酸拉链和跨膜结构域，推测SPAG9介导的信号转导途径参与生殖过程。
