Chin Hee-Seung, Park Tae-Sung, Moon Yeon-Sung, Oh Jung-Hyub
Department of Ophthalmology, Inha University Hospital, Inchon, South Korea.
Retina. 2005 Jul-Aug;25(5):556-60. doi: 10.1097/00006982-200507000-00002.
To study the difference in clearance of intravitreal triamcinolone acetonide quantitatively between vitrectomized and nonvitrectomized eyes.
Eighty-four eyes of 42 rabbits were divided in 2 groups: 42 right eyes underwent standard pars plana vitrectomy (vitrectomized group), and 42 left eyes were not operated on (nonvitrectomized group). All eyes received intravitreal injections with 0.1 mL (0.3 mg) of triamcinolone acetonide. Every 12 eyes were obtained by killing 6 rabbits 1, 2, 4, 7, 12, 20, or 30 days after intravitreal injection. Each eye was enucleated and immediately frozen at -70 degrees C. The frozen vitreous was prepared for measuring the concentration of triamcinolone acetonide. Triamcinolone acetonide was quantified by high-performance liquid chromatography and ultraviolet absorbance detection.
After 30 days, triamcinolone acetonide was detected only in 1 eye (0.22 microg/mL) in the vitrectomized group compared with 4 of 6 eyes (0.92 +/- 1.25 microg/mL) in the nonvitrectomized group. The coefficient of logarithmic regression was -0.12 in the vitrectomized group and -0.08 in the nonvitrectomized group. Triamcinolone acetonide decreased 1.5 times more rapidly in the vitrectomized group than in the nonvitrectomized group. The half-life of triamcinolone acetonide was 1.57 days in the vitrectomized group and 2.89 days in the nonvitrectomized group.
Intravitreal triamcinolone acetonide decreases more rapidly in the vitrectomized eye than in the nonvitrectomized eye. Therefore, the faster clearance of intravitreal triamcinolone acetonide must be considered when planning intravitreal injection of triamcinolone acetonide in the vitrectomized eye.
定量研究玻璃体切割眼与未行玻璃体切割眼玻璃体内注射曲安奈德后的清除差异。
42只兔的84只眼分为2组：42只右眼行标准的扁平部玻璃体切除术（玻璃体切割组），42只左眼未手术（未行玻璃体切割组）。所有眼均玻璃体内注射0.1 mL（0.3 mg）曲安奈德。在玻璃体内注射后1、2、4、7、12、20或30天，每组通过处死6只兔获取12只眼。每只眼摘除后立即在-70℃冷冻。将冷冻的玻璃体用于测量曲安奈德的浓度。采用高效液相色谱法和紫外吸收检测法定量曲安奈德。
30天后，玻璃体切割组仅在1只眼中检测到曲安奈德（0.22μg/mL），而未行玻璃体切割组6只眼中有4只眼检测到（0.92±1.25μg/mL）。玻璃体切割组对数回归系数为-0.12，未行玻璃体切割组为-0.08。玻璃体切割组曲安奈德下降速度比未行玻璃体切割组快1.5倍。玻璃体切割组曲安奈德的半衰期为1.57天，未行玻璃体切割组为2.89天。
玻璃体内注射曲安奈德后，玻璃体切割眼比未行玻璃体切割眼清除更快。因此，在计划对玻璃体切割眼进行玻璃体内注射曲安奈德时，必须考虑到玻璃体内曲安奈德清除更快这一因素。
