Hawthorne Clementine M, Farber Phillip J, Bibbo Marluce
Cytology Laboratory, Thomas Jefferson University Hospital, Philadelphia, Pennsylvania 19107, USA.
Diagn Cytopathol. 2005 Sep;33(3):177-80. doi: 10.1002/dc.20325.
Residual material from liquid-based pap (LBP) test has been used for molecular testing of Human Papilloma Virus (HR HPV) DNA. LBP test specimens (1,025) were evaluated to determine how often residual material was insufficient to perform microbiological testing. The specimens were tested for Chlamydia trachomatis and Neisseria gonorrhea (CCGT) alone and in combination with HR HPV(CCGT + HR HPV), using PCR method and HYBRID CAPTURE 2, respectively. A minimum volume of 5 ml (CCGT: 0.5-1 ml, HPV: 4 ml) of residual material is required for both the tests. The residual material was measured at >5 ml, < or =5 ml, and 0.5 ml volumes and documented. Results were tabulated. The insufficient rate for cases submitted for CCGT was 0.2% and 7.6% for HR HPV, with a detection rate of 40.9% for HR HPV. Utilizing residual material from LBP test for microbiological testing, as well as DNA testing for HR HPV is a convenient, cost-effective means to enhance patient care.
液基薄层涂片（LBP）检测的剩余样本已用于人乳头瘤病毒（HR HPV）DNA的分子检测。对1025份LBP检测样本进行评估，以确定剩余样本不足以进行微生物检测的频率。分别采用PCR方法和杂交捕获2法对样本单独检测沙眼衣原体和淋病奈瑟菌（CCGT），以及联合检测CCGT和HR HPV（CCGT + HR HPV）。两种检测均至少需要5毫升（CCGT：0.5 - 1毫升，HPV：4毫升）的剩余样本。测量剩余样本体积大于5毫升、小于或等于5毫升以及0.5毫升，并记录相关数据。结果制成表格。提交CCGT检测的样本不足率为0.2%，HR HPV为7.6%，HR HPV的检测率为40.9%。利用LBP检测的剩余样本进行微生物检测以及HR HPV的DNA检测，是一种方便且具成本效益的提升患者护理的方法。
