Riyazi Naghmeh, Kurreeman Fina A S, Huizinga Tom W J, Dekker Friedo W, Stoeken-Rijsbergen Gerrie, Kloppenburg Margreet
Department of Rheumatology and Clinical Epidemiology, Leiden University Medical Center, The Netherlands.
J Rheumatol. 2005 Aug;32(8):1571-5.
The interleukin (IL)-10 single nucleotide promoter polymorphism (SNP) -2849A is associated with decreased IL-10 production as measured by lipopolysaccharide (LPS) stimulated whole blood cultures. A low innate production of IL-10 using the same assay is associated with an increased risk of familial osteoarthritis (OA). We investigated the association of 7 novel SNP located downstream of the IL-10 transcription start site: -2849,-2763, -1330, -1082, -819, and -592, constituting the 4 ancient haplotypes, with distal interphalangeal (DIP) OA.
The study population comprised consecutive patients with and without radiological DIP OA (Kellgren-Lawrence score of > or = 2 in one joint) aged 40-70 years from a cohort of subjects with different types of arthritis in an early stage referred to an Early Arthritis Clinic (EAC). DNA typing for IL-10 SNP as well as radiographs of the hands were performed at clinic enrolment. Patients with rheumatoid arthritis, systemic lupus erythematosus, spondyloarthropathies, and psoriatic arthritis were excluded.
The distribution of DIP OA and IL-10 SNP were comparable to representative samples of the Dutch population. In the cohort of 172 subjects, 57 had DIP OA (33%) and 115 (67%) had no DIP OA. No significant association was found between DIP OA and IL-10 SNP and the 4 common haplotypes IL10.1, IL10.2, IL10.3, and IL10.4.
Our data suggest that IL-10 SNP, including -2849, which is associated with differential production, do not play a major role in the susceptibility of DIP OA.
白细胞介素（IL）-10单核苷酸启动子多态性（SNP）-2849A与脂多糖（LPS）刺激全血培养物所测得的IL-10产生减少相关。使用相同检测方法时，IL-10的先天性低产生与家族性骨关节炎（OA）风险增加相关。我们研究了位于IL-10转录起始位点下游的7个新型SNP：-2849、-2763、-1330、-1082、-819和-592（构成4种古老单倍型）与远端指间关节（DIP）OA的关联。
研究人群包括来自早期关节炎诊所（EAC）的不同类型关节炎早期队列中年龄40至70岁、有或无放射学DIP OA（一个关节的Kellgren-Lawrence评分≥2）的连续患者。在诊所入组时进行IL-10 SNP的基因分型以及手部X线片检查。排除类风湿关节炎、系统性红斑狼疮、脊柱关节炎和银屑病关节炎患者。
DIP OA和IL-10 SNP的分布与荷兰人群的代表性样本相当。在172名受试者队列中，57人有DIP OA（33%），115人（67%）无DIP OA。未发现DIP OA与IL-10 SNP以及4种常见单倍型IL10.1、IL10.2、IL10.3和IL10.4之间存在显著关联。
我们的数据表明，包括与产生差异相关的-2849在内的IL-10 SNP在DIP OA易感性中不发挥主要作用。
