Quarino Lawrence, Dang Qui, Hartmann John, Moynihan Nora
Office of Chief Medical Examiner, Department of Forensic Biology, New York City, USA.
J Forensic Sci. 2005 Jul;50(4):873-6.
An ELISA method for the detection of salivary amylase in dried stains using a monoclonal anti-human salivary amylase antibody was developed. Studies demonstrated the assay to be sensitive down to 0.0002 Sigma units and showed a linear response between absorbance and salivary amylase activity between 0.002 and 0.2 units. The assay showed no cross reactivity with either commercially purchased human pancreatic or bacterial amylase. Sample studies utilizing swabs from several human body fluids showed that 100% of all saliva containing swabs (sixteen of sixteen) and 13% of non-saliva human body fluid swabs (eight of sixty-three) showed a net absorbance with the method. Of these eight non-saliva swabs yielding a net absorbance, none exceeded a salivary amylase activity of 0.003 units. In contrast, only three of the sixteen saliva-containing swabs (swabs produced from saliva diluted 1:5, 1:6, and 1:10, respectively) showed an activity below 0.2 units. Of these swabs, the 1:100 dilution showed the lowest activity (0.048). This value is still more than ten times that of the non-saliva containing swab with the highest activity.
开发了一种使用单克隆抗人唾液淀粉酶抗体检测干污渍中唾液淀粉酶的ELISA方法。研究表明该测定法对低至0.0002西格玛单位的唾液淀粉酶敏感，并且在0.002至0.2单位之间吸光度与唾液淀粉酶活性呈线性响应。该测定法与市售的人胰腺淀粉酶或细菌淀粉酶均无交叉反应。利用来自几种人体体液的拭子进行的样本研究表明，所有含唾液的拭子（16个中的16个）中有100%以及13%的非唾液人体体液拭子（63个中的8个）用该方法显示出净吸光度。在这8个产生净吸光度的非唾液拭子中，没有一个超过0.003单位的唾液淀粉酶活性。相比之下，16个含唾液的拭子中只有3个（分别由稀释1:5、1:6和1:10的唾液产生的拭子）显示活性低于0.2单位。在这些拭子中，1:100稀释度显示出最低活性（0.048）。该值仍比活性最高的非唾液含拭子的值高十多倍。
