Daurai Bethuel, Gogoi Manashjit
Department of Biomedical Engineering, North-Eastern Hill University Shillong Meghalaya India
RSC Adv. 2024 Aug 5;14(34):24365-24372. doi: 10.1039/d4ra03804e.
Pancreatitis is a life-threatening inflammatory disease of the pancreas. In 2019, 34.8 out of 100 000 people suffered from acute pancreatitis globally. In humans, the level of α-amylase increases three times the normal value during pancreatitis. α-Amylase is an enzyme that hydrolyses α-1,4 glycosidic bonds of starch. In this study, we investigated a novel distance-based sensing method. We exploited the existing starch triiodide method, where the blue colour of starch-triiodide fades away and becomes colourless when α-amylase breaks the starch chain at the α-1-4 glycosidic bond. A hydrophilic channel was made on paper using a simple laser printer to create hydrophobic barriers. This channel was impregnated with starch triiodide, where α-amylase can turn it colourless. This distance covered by the change in colour is directly proportional to the concentration of α-amylase in a sample. Simulated samples with different concentrations of porcine α-amylase and pancreatin were used for testing using the developed paper-based chemometer device. The paper-based chemometer device was also tested with artificial blood serum with different concentrations of α-amylase. The of this device was found to be 0.9905, and the accuracy of the device when compared with a 2-chloro-4-nitrophenyl-α-d-maltotrioside method was found to be 95.54% with a sensitivity of 0.131 U L mm. Correlation test also showed that the paper-based chemometer device for α-amylase can be used as a testing device for artificial blood serum. This is a preliminary investigation that shows promising results. The chemometer devices stored in air-tight packets at 4-8 °C in a refrigerator did not lose the colour intensity until day 90 and retained an accuracy of 94.5%. However, the device needs to be evaluated in clinical settings prior to using it for measuring α-amylase in patients.
胰腺炎是一种危及生命的胰腺炎症性疾病。2019年，全球每10万人中有34.8人患有急性胰腺炎。在人类中，胰腺炎期间α淀粉酶水平会升至正常值的三倍。α淀粉酶是一种水解淀粉α-1,4糖苷键的酶。在本研究中，我们研究了一种新型的基于距离的传感方法。我们利用了现有的淀粉三碘化物法，即当α淀粉酶在α-1,4糖苷键处打断淀粉链时，淀粉三碘化物的蓝色会褪去并变为无色。使用简单的激光打印机在纸上制作一个亲水通道以形成疏水屏障。该通道用淀粉三碘化物浸渍，α淀粉酶可使其变为无色。颜色变化所覆盖的距离与样品中α淀粉酶的浓度成正比。使用不同浓度猪α淀粉酶和胰酶的模拟样品对所开发的纸质化学计量器装置进行测试。该纸质化学计量器装置也用不同浓度α淀粉酶的人工血清进行了测试。该装置的 为0.9905，与2-氯-4-硝基苯基-α-D-麦芽三糖苷法相比，该装置的准确度为95.54%，灵敏度为0.131 U/L/mm。相关性测试还表明，用于α淀粉酶的纸质化学计量器装置可作为人工血清的检测装置。这是一项显示出有前景结果的初步研究。储存在冰箱中4-8°C气密包装中的化学计量器装置直到第90天颜色强度都没有损失，准确度保持在94.5%。然而，在将该装置用于测量患者的α淀粉酶之前，需要在临床环境中进行评估。
