Li Li, Li Zi-cheng, Yan Heng-xiu, Hu Hai-yan, Chen Li, Yang Wen-xing, Zheng Yu
Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Jul;36(4):533-6.
To study the protective effect of tetramethylpyrazine (TMP) on the respiratory inhibition induced by hypoxia.
Forty-four SD rats were used. The hypoxic rat model was established. The effects of TMP on the responses of respiratory activity and life time to hypoxia were observed. The Nissl's staining and immunohistochemical technique were used to investigate the effect of hypoxia on the Nissl's bodies and FOS expression in the brainstem and the antagonistic action of TMP.
In contrast to the group of hypoxia, the occurrence of respiratory inhibition was delayed and the life time was prolonged obviously in the rats of hypoxia plus TMP (P<0.01). Compared with the air contronl group, the intensity of the Nissl's staining of the brainstem neurons became weaker in the group of hypoxia, but in the group of hypoxia plus TMP, the Nissl's staining was stronger and the gray degree decreased obviously, compared with the group of hypoxia (P<0.05). FOS positive neurons were induced by hypoxia and mainly existed in the nucleus of solitary tract, area postrema, hypoglossal nucleus, lateral reticular nucleus, inferior olivary nucleus, nucleus raphe pallidus, facial nucleus, trapezoid nucleus, but in the group of hypoxia plus TMP, the level of FOS immunoreactivity decreased remarkably, compared with the group of hypoxia (P<0.05).
TMP has protective effect on the respiratory inhibition induced by hypoxia and FOS may be involved in the protection.
研究川芎嗪（TMP）对缺氧诱导的呼吸抑制的保护作用。
采用44只SD大鼠，建立缺氧大鼠模型，观察TMP对缺氧时呼吸活动反应及存活时间的影响，应用尼氏染色和免疫组化技术观察缺氧对脑干尼氏体及FOS表达的影响以及TMP的拮抗作用。
与缺氧组相比，缺氧加TMP组大鼠呼吸抑制出现延迟，存活时间明显延长（P<0.01）。与空气对照组相比，缺氧组脑干神经元尼氏染色强度减弱，而缺氧加TMP组尼氏染色增强，灰度明显降低，与缺氧组相比差异有统计学意义（P<0.05）。缺氧诱导FOS阳性神经元，主要存在于孤束核、最后区、舌下神经核、外侧网状核、下橄榄核、中缝苍白核、面神经核、斜方体核，但缺氧加TMP组与缺氧组相比，FOS免疫反应性水平明显降低（P<0.05）。
TMP对缺氧诱导的呼吸抑制有保护作用，FOS可能参与了该保护作用。
