Li Li, Gao Bo, Liu Meifang
School of Pharmacy, Jining Medical University, Rizhao 276826, China.
Evid Based Complement Alternat Med. 2020 Sep 29;2020:6456017. doi: 10.1155/2020/6456017. eCollection 2020.
Tetramethylpyrazine (TMP) is an alkaloid extracted from the root and stem of the traditional Chinese herbal medicine called Chuanxiong. The present study aims to study the effects of TMP on hypoxic respiratory depression in rats. . The effects of TMP on respiratory responses of rats induced by hypoxia were observed by diaphragm electromyogram (EMG) recording. The effects of TMP on the protein expression of FOS and acid sensing ion channel (ASIC) in the brainstem induced by hypoxia were investigated by immunohistochemistry.
The respiration of rats was first excited and then depressed during hypoxia treatment, while TMP pretreatment could significantly antagonize the respiratory depression induced by hypoxia ( < 0.01). Hypoxia obviously induced the protein expression of FOS ( < 0.01) and ASIC( < 0.05) in the brainstem, which can be also significantly inhibited by TMP pretreatment.
TMP has protective effects on hypoxic respiratory depression, and the mechanisms might be concerned with its downregulation of FOS and ASIC in the brainstem induced by hypoxia.
川芎嗪（TMP）是从传统中草药川芎的根茎中提取的一种生物碱。本研究旨在探讨川芎嗪对大鼠缺氧性呼吸抑制的影响。通过膈肌肌电图（EMG）记录观察川芎嗪对缺氧诱导的大鼠呼吸反应的影响。采用免疫组织化学法研究川芎嗪对缺氧诱导的脑干中FOS和酸敏感离子通道（ASIC）蛋白表达的影响。
缺氧处理期间大鼠呼吸先兴奋后抑制，而川芎嗪预处理可显著拮抗缺氧诱导的呼吸抑制（<0.01）。缺氧明显诱导脑干中FOS（<0.01）和ASIC（<0.05）蛋白表达，川芎嗪预处理也可显著抑制。
川芎嗪对缺氧性呼吸抑制具有保护作用，其机制可能与其下调缺氧诱导的脑干中FOS和ASIC有关。
