Yu Qiong-Wei, Lin Bo, He Hai-Bo, Shi Zhi-Guo, Feng Yu-Qi
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
J Chromatogr A. 2005 Aug 12;1083(1-2):23-31. doi: 10.1016/j.chroma.2005.05.104.
A novel immobilization method was proposed for the preparation of pyrenebutyric acid-bonded silica (PYB-silica) stationary phases. The pyrene moiety was grafted to silica gel through spacers of aminoalkyl silanes. The HPLC separation of C60, C70 and higher fullerenes on the new pyrenebutyric acid-bonded silica stationary phases was also studied. Based on the temperature effect, the intermolecular interaction between stationary phases and solutes and the retention mechanism were discussed. The results of column loading capacity test demonstrated the potential for the separation of fullerenes in large amounts on the PYB-silica stationary phases.
提出了一种用于制备芘丁酸键合硅胶（PYB-硅胶）固定相的新型固定化方法。芘部分通过氨基烷基硅烷间隔基接枝到硅胶上。还研究了在新型芘丁酸键合硅胶固定相上对C60、C70及更高富勒烯的高效液相色谱分离。基于温度效应，讨论了固定相与溶质之间的分子间相互作用及保留机理。柱负载量测试结果表明了在PYB-硅胶固定相上大量分离富勒烯的潜力。
