School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
The John van Geest Cancer Research Centre, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
Int J Mol Sci. 2021 May 27;22(11):5726. doi: 10.3390/ijms22115726.
A silica-bound -butylpyrogallol[4]arene chromatographic stationary phase was prepared and characterised by thermogravimetric analysis, scanning electron microscopy, NMR and mass spectrometry. The chromatographic performance was investigated by using C and C fullerenes in reverse phase mode via flash column and high-pressure liquid chromatography (HPLC). The resulting new stationary phase was observed to demonstrate size-selective molecular recognition as postulated from our in-silico studies. The silica-bound -butylpyrogallol[4]arene flash and HPLC stationary phases were able to separate a C- and C-fullerene mixture more effectively than an RP-C stationary phase. The presence of toluene in the mobile phase plays a significant role in achieving symmetrical peaks in flash column chromatography.
一种键合在硅胶上的 -丁基苯并[g,h,i]苝色谱固定相通过热重分析、扫描电子显微镜、NMR 和质谱进行了制备和表征。通过反相模式的闪蒸柱和高效液相色谱（HPLC），使用 C 和 C 富勒烯对色谱性能进行了研究。从我们的计算机模拟研究中可以推断出，所得的新型固定相表现出尺寸选择性的分子识别。硅胶键合的 -丁基苯并[g,h,i]苝闪蒸和 HPLC 固定相比 RP-C 固定相更有效地分离 C 和 C 富勒烯混合物。流动相中甲苯的存在对在闪蒸柱色谱中获得对称峰起着重要作用。
