采用程序场强梯度的微芯片毛细管凝胶电泳用于大豆中转基因生物的超快速分析。

Microchip capillary gel electrophoresis using programmed field strength gradients for the ultra-fast analysis of genetically modified organisms in soybeans.

作者信息

Kim Yun-Jeong, Chae Joon-Seok, Chang Jun Keun, Kang Seong Ho

机构信息

Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea.

出版信息

J Chromatogr A. 2005 Aug 12;1083(1-2):179-84. doi: 10.1016/j.chroma.2005.06.002.

DOI:10.1016/j.chroma.2005.06.002

PMID:16078705

Abstract

We have developed a novel method for the ultra-fast analysis of genetically modified organisms (GMOs) in soybeans by microchip capillary gel electrophoresis (MCGE) using programmed field strength gradients (PFSG) in a conventional glass double-T microchip. Under the programmed electric field strength and 0.3% poly(ethylene oxide) sieving matrix, the GMO in soybeans was analyzed within only 11 s of the microchip. The MCGE-PFSG method was a program that changes the electric field strength during GMO analysis, and was also applied to the ultra-fast analysis of PCR products. Compared to MCGE using a conventional and constantly applied electric field, the MCGE-PFSG analysis generated faster results without the loss of resolving power and reproducibility for specific DNA fragments (100- and 250-bp DNA) of GM-soybeans. The MCGE-PFSG technique may prove to be a new tool in the GMO analysis due to its speed, simplicity, and high efficiency.

摘要

我们开发了一种新方法，可通过在传统玻璃双T型微芯片中使用程序场强梯度（PFSG）的微芯片毛细管凝胶电泳（MCGE）对大豆中的转基因生物（GMO）进行超快速分析。在程序电场强度和0.3%聚环氧乙烷筛分基质条件下，仅需11秒即可在微芯片中对大豆中的转基因生物进行分析。MCGE-PFSG方法是一种在转基因生物分析过程中改变电场强度的程序，也应用于PCR产物的超快速分析。与使用传统恒定电场的MCGE相比，MCGE-PFSG分析产生结果的速度更快，同时不会损失转基因大豆特定DNA片段（100和250碱基对DNA）的分辨能力和重现性。由于其速度快、操作简单且效率高，MCGE-PFSG技术可能成为转基因生物分析中的一种新工具。