Azuhata F, Uyeda I, Shikata E
Department of Botany, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
J Gen Virol. 1992 Jun;73 ( Pt 6):1593-5. doi: 10.1099/0022-1317-73-6-1593.
The terminal regions of the dsRNA genome segments of rice black streaked dwarf virus (RBSDV) were sequenced. The individual dsRNAs, which were 32P-labelled at their 3' termini by incubation with [32P]pCp and T4RNA ligase, were separated by 5% PAGE, and the 10 dsRNA segments were sequenced by two-dimensional electrophoresis. The common 3'-terminal sequences ---GUC 3' and ---AAAAACUU 3' were found in the plus and minus strands, respectively. The strictly conserved terminal sequences (5' AAGUUUUU ... GUC 3') of the genome segments of RBSDV differ from those of the phytoreoviruses and rice ragged stunt virus.
对水稻黑条矮缩病毒（RBSDV）双链RNA基因组片段的末端区域进行了测序。通过与[32P]pCp和T4 RNA连接酶孵育，在其3'末端用32P标记的各个双链RNA，经5%聚丙烯酰胺凝胶电泳（PAGE）分离，然后通过二维电泳对10个双链RNA片段进行测序。在正链和负链中分别发现了共同的3'-末端序列---GUC 3'和---AAAAACUU 3'。RBSDV基因组片段严格保守的末端序列（5' AAGUUUUU...GUC 3'）与植物呼肠孤病毒和水稻锯齿叶矮缩病毒的末端序列不同。
