Khozhaĭ L I
Morfologiia. 2005;127(1):17-21.
Time-course of astrocyte appearance in the neocortex of F1 (CBA/C57B1) mice after prenatal serotonin depletion was studied. To suppress serotonin synthesis, para-chlorophenylalanine was administered to females once at the early postimplantation stage of gestation. To visualize differentiating astrocytes, the immunohistochemical method for demonstration of astrocyte intermediate filament protein--glial fibrillary acidic protein (GFAP) was used, which allowed not only to label the cells, but also to evaluate the starting point, extent and time-course of their differentiation. The results obtained have shown that in all the neocortical areas studied (area cingularis, area occipitalis, area parietalis, area insularis, area praepiriformis, area piriformis, area entorhinalis and area subiculum), normally, GFAP-positive cells appeared in the layer I at postnatal week 1. In the course of development, the process of astrocyte differentiation was shown to become progressively more intensive. At the same time, the process of barrier structures formation took place. During the first days of postnatal development astrocytes and their processes were shown to appear close to the blood vessels and lateral ventricle walls. In the neocortex of animals that developed during the serotonin depletion, GFAP-positive cells were formed in decreased numbers, in particular, in the white matter, at all the stages of postnatal development studied.
研究了产前血清素耗竭后F1（CBA/C57B1）小鼠新皮质中星形胶质细胞出现的时间进程。为了抑制血清素合成，在妊娠植入后早期给雌性小鼠一次性注射对氯苯丙氨酸。为了可视化分化中的星形胶质细胞，使用了免疫组织化学方法来显示星形胶质细胞中间丝蛋白——胶质纤维酸性蛋白（GFAP），这不仅可以标记细胞，还可以评估其分化的起始点、程度和时间进程。所得结果表明，在所有研究的新皮质区域（扣带回区域、枕叶区域、顶叶区域、岛叶区域、梨状前区域、梨状区域、内嗅区域和下托区域），正常情况下，GFAP阳性细胞在出生后第1周出现在I层。在发育过程中，星形胶质细胞的分化过程显示出逐渐增强。与此同时，屏障结构的形成过程也在发生。在出生后发育的最初几天，星形胶质细胞及其突起显示出出现在靠近血管和侧脑室壁的位置。在血清素耗竭期间发育的动物的新皮质中，在所有研究的出生后发育阶段，GFAP阳性细胞的形成数量减少，尤其是在白质中。
