Petrova E S
Morfologiia. 2011;139(2):22-6.
The purpose of this study was to investigate the expression of intermediate filament proteins (vimentin and glial fibrillary acidic protein--GFAP) in the cells of embryonic rat neocortex at different time points after its allotransplantation into injured sciatic nerve of adult animals. Using immunohistochemical methods, the differentiation of GFAP-positive astrocytes from vimentin-positive radial glial cells was observed in embryonic rat neocortex, grafted into sciatic nerve. It was shown that the differentiation of the embryonic neocortical astrocytes in the transplants took place a few days earlier than in the rat neocortex during normal ontogenesis. Reactive gliosis was demonstrated in the long-term transplants, as indicated by a large number of intensely stained GFAP-positive cells and vimentin-containing astrocytes. These findings suggest that ectopic neurotarnsplants could serve as a model for fundamental studies of the mechanisms of reactive gliosis development.
本研究的目的是调查胚胎大鼠新皮质细胞中中间丝蛋白（波形蛋白和胶质纤维酸性蛋白——GFAP）在同种异体移植到成年动物损伤坐骨神经后不同时间点的表达情况。采用免疫组织化学方法，在移植到坐骨神经的胚胎大鼠新皮质中观察到波形蛋白阳性的放射状胶质细胞分化为GFAP阳性的星形胶质细胞。结果表明，移植中胚胎新皮质星形胶质细胞的分化比正常个体发育过程中大鼠新皮质的分化提前了几天。长期移植中出现了反应性胶质增生，表现为大量强染色的GFAP阳性细胞和含波形蛋白的星形胶质细胞。这些发现表明，异位神经移植可作为反应性胶质增生发生机制基础研究的模型。
