Al-Maghrebi May, Anim Jeroham T, Olalu Aaron A
Department of Biochemistry, Faculty of Medicine, Kuwait University.
Anticancer Res. 2005 May-Jun;25(3c):2573-7.
Wide evidence suggests the involvement of translation elongation factors (EFs) at the onset of oncogenesis. To investigate the potential role of the EF-1 subunits (A, Balpha and Bgamma) in the formation and progression of breast cancer, we quantified their expression in human breast tissues and cell lines.
The mRNA levels of EF-1A, -1Balpha and -1Bgamma in human breast tissues and cell lines were measured by semi-quantitative RT-PCR and Northern blotting, respectively.
The mRNA expression of the three EF-1 subunits was significantly higher in cancerous over normal tissues. However, there was no significant difference in their expression between tumor grades. Overexpression of EF-1 mRNA in breast cancer cell lines were not caused by increased mRNA stability.
Although not indicative of tumor grading, the elevated levels of EF-1 subunits are suggestive of their early role in the pathogenesis of breast cancer, possibly through their increased rate of transcription.
大量证据表明翻译延伸因子（EFs）在肿瘤发生起始阶段发挥作用。为研究EF-1亚基（A、Bα和Bγ）在乳腺癌形成和进展中的潜在作用，我们对其在人乳腺组织和细胞系中的表达进行了定量分析。
分别采用半定量逆转录聚合酶链反应（RT-PCR）和Northern印迹法检测人乳腺组织和细胞系中EF-1A、-1Bα和-1Bγ的mRNA水平。
三种EF-1亚基的mRNA在癌组织中的表达显著高于正常组织。然而，它们在不同肿瘤分级之间的表达无显著差异。乳腺癌细胞系中EF-1 mRNA的过表达并非由mRNA稳定性增加所致。
虽然EF-1亚基水平升高与肿瘤分级无关，但提示其可能在乳腺癌发病机制早期发挥作用，可能是通过增加转录速率实现的。
