[白细胞介素-1α对兔眼眼压的影响及其局部副作用]

[The effect of interleukin-1alpha on intraocular pressure in rabbit eyes and its focal side effects].

作者信息

Wang Ning-li, Chen Jing-chang, Fan Zhi-gang, Wu He-ping, Lin Hui, Qiao Li-ya

机构信息

Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

出版信息

Zhonghua Yan Ke Za Zhi. 2005 Jul;41(7):647-51.

PMID:16080902

Abstract

OBJECTIVE

To investigate the efficiency of interleukin-1alpha on intraocular pressure reduction and its safety.

METHODS

35 New Zealand female rabbits were randomized into seven groups. In group-A, one eye was randomly selected to receive sub-conjunctival injection of 15 ng IL-1alpha, and in group B-D, one eye was injected intracamerally with IL-1alpha 1.5 ng, 15 ng and 40 ng respectively, and the other eye was injected intracamerally with equivalent volume of 0.1% PBS as control. In group-E, one eye was treated with 0.5% timolol eye drops and the other eye was given artificial tear. Groups F and G were treated as group D, and specifically in group-G, both eyes were given IL-1alpha. In group-A to D, the examination of tonometry, slit-lamp biomicroscopy and direct ophthalmoscope were taken before treatment and 24 hours after the treatment and were repeated everyday for 4 days. In group-E, tonometry was applied before treatment and 7 days after treatment. In group-F, aqueous humor of two eyes was aspirated for smear examination 24 and 48 hours after treatment, and corneal endothelium microscope and flash-ERG were done in group-G before and 30 to 40 hours after treatment, and then the eyes were enucleated for histology analysis.

RESULTS

IOP of the eyes received IL-1alpha was decreased significantly compared with that of contralateral control eyes in group A to D (P < 0.05). Peaking time of IOP reduction was 72 - 96 hours after treatment, and IOP reduction was continued for over 96 hours. The IOP reduction efficiency in group C and D was more significant than those of group E (P < 0.05). No significant abnormal was found with the examinations of slit-lamp biomicroscopy, ophthalmoscope, corneal endothelium microscopy, flash-ERG, smear and histological observation, except for mild focal conjunctival congestion.

CONCLUSION

Intraocular reduction were demonstrated with IL-1alpha treatment, charactered by its strong hypotensive effect, long duration and safety.

摘要

目的

研究白细胞介素-1α降低眼压的效果及其安全性。

方法

将35只新西兰雌性家兔随机分为7组。A组中，随机选择一只眼结膜下注射15 ng白细胞介素-1α，B-D组中，一只眼分别前房内注射1.5 ng、15 ng和40 ng白细胞介素-1α，另一只眼前房内注射等量的0.1% PBS作为对照。E组中，一只眼用0.5%噻吗洛尔滴眼液治疗，另一只眼给予人工泪液。F组和G组按D组治疗，具体而言，G组双眼均给予白细胞介素-1α。A-D组在治疗前和治疗后24小时进行眼压测量、裂隙灯显微镜检查和直接检眼镜检查，并连续4天每天重复检查。E组在治疗前和治疗后7天进行眼压测量。F组在治疗后24小时和48小时抽取双眼房水进行涂片检查，G组在治疗前和治疗后30至40小时进行角膜内皮显微镜检查和闪光视网膜电图检查，然后摘除眼球进行组织学分析。

结果

A-D组中，接受白细胞介素-1α治疗的眼眼压较对侧对照眼显著降低（P < 0.05）。眼压降低的峰值时间为治疗后72 - 96小时，眼压降低持续超过96小时。C组和D组的眼压降低效果比E组更显著（P < 0.05）。除轻度局限性结膜充血外，裂隙灯显微镜检查、检眼镜检查、角膜内皮显微镜检查、闪光视网膜电图检查、涂片检查和组织学观察均未发现明显异常。